NDLI: Simple LC–MS Determination of Citric and Malic Acids in Fruits and Vegetables

Content Provider	Springer Nature Link
Author	Fernández Fernández, Remedios López Martínez, Juan Carlos Romero González, Roberto Martínez Vidal, José Luis Alarcón Flores, María Isabel Garrido Frenich, Antonia
Copyright Year	2010
Abstract	A rapid, reliable and sensitive method has been developed to determine malic and citric acid in fruits and vegetables. The methodology is based on simple extraction with an aqueous solution of ethanol (80% v/v) and subsequent chromatographic analysis by liquid chromatography coupled to mass spectrometry. Electrospray ionization in negative mode was used. The best response for citric and malic acid was provided by molecular ions [M−H]− at m/z 191 and 133 respectively. These ions were used for quantification, whereas other fragments were used as confirmation ions. Different variables involved in the separation and detection process, such as mobile phase, gradient profile and flow rate have been optimised. Linearity, repeatability, recovery and limits of quantification were evaluated. Good linearity was obtained up to 5,000 mg kg−1. Recovery ranged from 90.0 to 104.6%, repeatability (expressed as RSD) was <8% for tested matrices, and limits of quantification were equal or lower than 65 mg kg−1. Finally, the method was applied to the analysis of samples of orange, tomato and pepper.
Starting Page	55
Ending Page	62
Page Count	8
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	72
Issue Number	1-2
e-ISSN	16121112
Language	English
Publisher	Vieweg Verlag
Publisher Date	2010-05-08
Publisher Place	Wiesbaden
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Column liquid chromatography–mass spectrometry Fruits and vegetables Citric acid Malic acid Measurement Science and Instrumentation Plant Sciences Biochemistry Pharmacy Organic Chemistry Analytical Chemistry
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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