NDLI: Effective Pre-Concentration and Analysis of Heavy Metals Using Ligand Step Gradient Focusing in Combination with Isotachophoresis

Content Provider	Springer Nature Link
Author	Glovivá, Eliška Pospíchal, Jan
Copyright Year	2012
Abstract	A capillary electrophoretic method for the pre-concentration of metals based on selective focusing of metal chelates with subsequent online ITP analysis was developed and verified. The selected ions of heavy metals (Pb2+ and Cd2+) were subjected to pre-concentration from the mixture and analysed. Focusing of the metals was carried out in a ligand step gradient, which was created by the addition of a convenient ligand agent to the regular stationary pH step gradient. The metal ions were continuously dosed into the column, where they were selectively trapped (with the exception of Na+) on the stationary ligand step gradient in the form of non-moving zones of citrate complexes with an effective charge of zero. After a detectable amount of analyte was accumulated, the dosing was stopped and the accumulated zones were mobilized to the analytical column, where they were analysed by the ITP method with conductivity or photometric detection. The electrolyte systems for the dosing (mode IEF), mobilizing (mode MBE), and analytical steps (mode ITP) were developed and tested. The cLOD was decreased by approximately two orders of magnitude using an acceptable dosing time of 25 min.
Starting Page	313
Ending Page	319
Page Count	7
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	76
Issue Number	7-8
e-ISSN	16121112
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-11-06
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Isotachophoresis Large volume pre-concentration Ligand step gradient focusing Metal chelate Heavy metals Selective pre-concentration Chromatography Proteomics Pharmacy Laboratory Medicine
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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