NDLI: The Effects of the Column Length on the Efficiency of Capillary Zwitterionic Organic Polymer Monolithic Columns in HILIC Chromatography

Content Provider	Springer Nature Link
Author	Jandera, Pavel Staňková, Magda
Copyright Year	2015
Abstract	Organic polymer monolithic columns of different lengths have been prepared in 320 µm i.d. fused silica capillary by in situ radical polymerization of N,N-dimethyl-N-methacryloxyethyl-N-(3-sulfopropyl) ammonium betaine as a zwitterionic functional monomer and bisphenol A glycerolate dimethacrylate as a crosslinking monomer in the presence of porogenic solvents. The zwitterionic monolithic columns are intended for separations of polar compounds in hydrophilic interaction chromatography (HILIC). The effects of the capillary column length, from 115 to 175 mm, on separation efficiency, were investigated under HILIC conditions, using 95:5 acetonitrile in water as the mobile phase. The extra-column contributions to band broadening significantly decrease the efficiency (apparent height equivalent to a theoretical plate), especially for weakly retained samples, and increase with diminishing column length. The experimental height equivalents of theoretical plate, HETP, were corrected for the extra-column contributions, which were determined for a series of columns by extrapolation to zero column length. On a 175 mm long column, the column efficiency, HETP = 16.5 μm, measured at the optimum linear flow velocity of 0.5 mm s−1, improved to HETP = 5 µm, after correction for extra-column contributions. For more strongly retained small polar compounds, interactions with zwitterionic groups and (or) water adsorbed inside the pores decrease the column efficiency at higher flow rates.
Starting Page	853
Ending Page	859
Page Count	7
File Format	PDF
ISSN	00095893
Journal	Chromatographia
Volume Number	78
Issue Number	13-14
e-ISSN	16121112
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-02-10
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Monolithic capillary columns HILIC Separation efficiency Column length Zwitterionic stationary phases Extra-column band broadening Chromatography Proteomics Pharmacy Laboratory Medicine
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Analytical Chemistry Biochemistry Clinical Biochemistry

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