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| Content Provider | Springer Nature Link |
|---|---|
| Author | Shahnazi, Sahar Meon, Sariah Vadamalai, Ganesan Ahmad, Khairulmazmi Nejat, Naghmeh |
| Copyright Year | 2012 |
| Abstract | Yellowing disease is one of the most important diseases of black pepper (Piper nigrum L.). To characterize the pathogen(s) responsible for yellowing disease of black pepper in Malaysia, 53 isolates of Fusarium were collected from the roots of diseased black pepper plants and from rhizosphere soils from major growing areas in Sarawak and Johor. A total of 34 isolates of F. solani and 19 isolates of F. proliferatum were obtained and identified based on morphological characteristics and molecular techniques. DNA sequencing of the internal transcribed spacers (ITS1 and ITS2) and 5.8S ribosomal DNA regions was conducted to identify Fusarium species. Nucleotide sequence analysis of the ITS regions revealed that this molecular technique enabled identification of Fusarium at the species level as F. solani and F. proliferatum. In a pathogenicity test on 3-month-old black pepper plants, F. solani was pathogenic, but F. proliferatum was not. On the basis of morphology, DNA sequences and pathogenicity of the fungal isolates from the diseased plants, we showed that yellowing disease on black pepper is caused by F. solani |
| Starting Page | 160 |
| Ending Page | 169 |
| Page Count | 10 |
| File Format | |
| ISSN | 13452630 |
| Journal | Journal of General Plant Pathology |
| Volume Number | 78 |
| Issue Number | 3 |
| e-ISSN | 1610739X |
| Language | English |
| Publisher | Springer Japan |
| Publisher Date | 2012-04-05 |
| Publisher Place | Japan |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Fusarium solani Yellowing disease Piper nigrum L. Black pepper Pathogenicity rDNA-ITS Microbiology Agriculture Plant Pathology |
| Content Type | Text |
| Resource Type | Book Review |
| Subject | Plant Science Agronomy and Crop Science |
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