NDLI: Response of transgenic cucumber expressing a rice class I chitinase gene to two fungal pathogens with different infectivities

Content Provider	Springer Nature Link
Author	Kishimoto, Kyutaro Nakajima, Masami Nishizawa, Yoko Tabei, Yutaka Hibi, Tadaaki Akutsu, Katsumi
Copyright Year	2003
Abstract	A transgenic cucumber line (CR32) over-expressing the rice class I chitinase gene exhibited resistance to Phytophthora rot (Phytophthora nicotianae var. parasitica) but not to Fusarium wilt (Fusarium oxysporum f. sp. cucumerinum). The infection behavior of these fungi on CR32 and nontransgenic plants was examined with an optical microscope. In zoosporangia of P. nicotianae var. parasitica, the rates of germination and penetration on leaves of both CR32 and nontransgenic plants were almost equal. After infection, however, the growth of infection hyphae was markedly suppressed in CR32 compared with their growth in the nontransgenic plants. In F. oxysporum f. sp. cucumerinum, the infection hyphae localized in petiole vessels of both CR32 and nontransgenic plants, and growth did not differ in the two plants. We investigated the antifungal activity of a high-molecular-weight fraction (HF) and a low-molecular-weight fraction (LF) of crude leaf extracts from CR32 and from the nontransgenic line. CR32 HF, which included the rice chitinase, had antifungal activity only against F. oxysporum f. sp. cucumerinum. In contrast, CR32 LF, which did not have the rice chitinase, had strong antifungal activity against the two fungi. These results suggested that a low-molecular-weight antifungal substance(s) was induced in CR32 and might function as a factor of resistance to P. nicotianae var. parasitica, which has cell walls that almost never contain chitin. Because rice chitinase has already been demonstrated not to localize in vessels of CR32, the infection localization of F. oxysporum f. sp. cucumerinum in vessels might enable the fungus to avoid antifungal substance(s), resulting in Fusarium wilt in transgenic cucumber.
Starting Page	358
Ending Page	363
Page Count	6
File Format	PDF
ISSN	13452630
Journal	Journal of General Plant Pathology
Volume Number	69
Issue Number	6
e-ISSN	1610739X
Language	English
Publisher	Springer-Verlag
Publisher Date	2003-12-18
Publisher Place	Tokyo
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cucumber Rice chitinase Phytophthora rot Fusarium wilt Resistance Antifungal substance
Content Type	Text
Resource Type	Article
Subject	Plant Science Agronomy and Crop Science

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