NDLI: A SCAR marker specific for rapid detection of the avirulence gene Avr1c in Phytophthora sojae

Content Provider	Springer Nature Link
Author	Wen, Jingzhi Chen, Qiuming Sun, Long Zhao, Liming Suo, Bing Tian, Miao
Copyright Year	2014
Abstract	The F2 population derived from a cross between isolates pRx (Avr1c-Avr1c) and ps1 (avr1c-avr1c) of Phytophthora sojae, fungal agent of soybean stem and root rot, was used to determine the genetic basis of avirulence towards Rps1c gene in soybean. The results indicated that this avirulence is dominant and controlled by a single locus, as expected for a simple gene-for-gene model. Segregation of Avr1c in the F2 progeny of this cross fits a 3:1 ratio. Four of 80 AFLP primers effectively distinguished the avirulent pRx from the virulent ps1. Among the 5 specific markers, band C was amplified from the avirulent pRx by primer set EGC/MAT, then recovered and cloned. This AFLP marker was successfully transfered to a SCAR marker through sequencing, primer design and specific amplication of the DNA of the avirulent pRx. Results of validity and specificity experiments with 50 individuals of the F2 progeny and 50 field isolates demonstrated that this SCAR marker (a 616-bp fragment) can be successfully and specifically amplified from the P. sojae isolates that have Avr1c gene.
Starting Page	415
Ending Page	422
Page Count	8
File Format	PDF
ISSN	13452630
Journal	Journal of General Plant Pathology
Volume Number	80
Issue Number	5
e-ISSN	1610739X
Language	English
Publisher	Springer Japan
Publisher Date	2014-05-29
Publisher Place	Tokyo
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Phytophthora sojae Avr1c SCAR marker Rapid detection Plant Pathology Agriculture Microbiology
Content Type	Text
Resource Type	Article
Subject	Plant Science Agronomy and Crop Science

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