NDLI: Genetic analysis and PCR-based identification of major Fusarium species causing head blight on wheat in Japan

Content Provider	Springer Nature Link
Author	Chung, Wen Hsin Ishii, Hideo Nishimura, Kumiko Ohshima, Michiyo Iwama, Toshitaka Yoshimatsu, Hideaki
Copyright Year	2008
Abstract	Identifying the Fusarium species cause Fusarium head blight (FHB) and produces mycotoxins in wheat and other cereal is difficult and time consuming because of confusing phenotypic classification systems. In Japan, the F. graminearum complex, F. culmorum, F. avenaceum, and Microdochium nivale predominantly cause FHB. The internal transcribed spacer (ITS) and 5.8S of rDNA, a partial sequence of β-tubulin and mitochondrial cytochrome b (cytb) genes of the four species were PCR-amplified and analyzed. On the basis of the ITS, β-tubulin and cytb sequences, F. avenaceum and M. nivale are distinct from the F. graminearum complex and F. culmorum, whereas the F. graminearum complex is closely related to F. culmorum. Moreover, thiophanate–methyl-resistant isolates of the F. graminearum complex and F. culmorum did not have an amino acid substitution at amino acid codon 198 or 200 of β-tubulin. In contrast, very highly or highly thiophanate–methyl-resistant isolates of M. nivale had Glu (GAG) substituted with Ala (GCG) or Lys (AAG) at codon 198, respectively. The allele-specific PCR assay was used to identify the F. graminearum complex and F. culmorum, and these Fusarium species could be distinguished rapidly.
Starting Page	364
Ending Page	374
Page Count	11
File Format	PDF
ISSN	13452630
Journal	Journal of General Plant Pathology
Volume Number	74
Issue Number	5
e-ISSN	1610739X
Language	English
Publisher	Springer Japan
Publisher Date	2008-08-09
Publisher Place	Japan
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Fusarium head blight rDNA-ITS β-tubulin Cytochrome b Allele-specific PCR Microbiology Agriculture Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Plant Science Agronomy and Crop Science

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