NDLI: Isolation of pathogenicity- and gregatin-deficient mutants of Phialophora gregata f. sp. adzukicola through Agrobacterium tumefaciens-mediated transformation

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Isolation of pathogenicity- and gregatin-deficient mutants of Phialophora gregata f. sp. adzukicola through Agrobacterium tumefaciens-mediated transformation

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Isolation of pathogenicity- and gregatin-deficient mutants of Phialophora gregata f. sp. adzukicola through Agrobacterium tumefaciens-mediated transformation

Content Provider	Springer Nature Link
Author	Tanaka, Soichi Kondo, rio Naito, Shigeo
Copyright Year	2007
Abstract	Phialophora gregata f. sp. adzukicola, a causal agent of brown stem rot in adzuki beans, produces phytotoxic compounds: gregatins A, B, C, D, and E. Gregatins A, C, and D cause wilting and vascular browning in adzuki beans, which resemble the disease symptoms. Thus, gregatins are considered to be involved in pathogenicity. However, molecular analyses have not been conducted, and little is known about other pathogenic factors. We sought to isolate nonpathogenic and gregatin-deficient mutants through Agrobacterium tumefaciens-mediated transformation (ATMT) for cloning of pathogenicity-related genes. The co-cultivation of P. gregata and A. tumefaciens for 48 h at 20°C with 200 μM acetosyringone resulted in approximately 80 transformants per 106 conidia. The presence of acetosyringone in the A. tumefaciens pre-cultivation period led to an increase in T-DNA copy number per genome. Of 420 and 110 transformants tested for their pathogenicity and productivity of gregatins, one nonpathogenic and three gregatin-deficient mutants were obtained, respectively. The nonpathogenic mutant produced gregatins, whereas the gregatin-deficient mutants had pathogenicity comparable to the wild-type strain. This is the first report of ATMT of P. gregata. Further analysis of these mutants will help reveal the nature of the pathogenicity of this fungus including the role of gregatin in pathogenesis.
Starting Page	242
Ending Page	249
Page Count	8
File Format	PDF
ISSN	13452630
Journal	Journal of General Plant Pathology
Volume Number	73
Issue Number	4
e-ISSN	1610739X
Language	English
Publisher	Springer-Verlag
Publisher Date	2007-07-05
Publisher Place	Tokyo
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Phialophora gregata Agrobacterium tumefaciens-mediated transformation Adzuki bean Gregatin Microbiology Agriculture Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Plant Science Agronomy and Crop Science

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New virulent race of Phialophora gregata f. sp. adzukicola associated with continuous cultivation of adzuki bean cultivar Acc259

Production of desmethyl-gregatin A, a possible causative toxin of brown stem rot in adzuki bean, by Phialophora gregata f. sp. adzukicola.

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New race of Phytophthora vignae f. sp. adzukicola, the causal agent of Phytophthora stem rot of the adzuki bean

Isolation of pathogenicity- and gregatin-deficient mutants of Phialophora gregata f. sp. adzukicola through Agrobacterium tumefaciens-mediated transformation

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