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| Content Provider | Springer Nature Link |
|---|---|
| Author | Mohammadian, Mahdi Fathi Roudsari, Mehrosh Mollania, Nasrin Badoei Dalfard, Arastoo Khajeh, Khosro |
| Copyright Year | 2010 |
| Abstract | Laccases (benzenediol oxygen oxidoreductase; EC 1.10.3.2) have many biotechnological applications because of their oxidation ability towards a wide range of phenolic compounds. Within recent years, researchers have been highly interested in the identification and characterization of laccases from bacterial sources. In this study, we have isolated and cloned a gene encoding laccase (CotA) from Bacillus sp. HR03 and then expressed it under microaerobic conditions and decreased temperature in order to obtain high amounts of soluble protein. The laccase was purified and its biochemical properties were investigated using three common laccase substrates, 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), syringaldazine (SGZ) and 2,6-dimethoxyphenol (2,6-DMP). K M and k cat were calculated 535 μM and 127 s−1 for ABTS, 53 μM and 3 s−1 for 2, 6-DMP and 5 μM and 20 s−1 for SGZ when the whole reactions were carried out at room temperature. Laccase activity was also studied when the enzyme was preincubated at 70 and 80°C. With SGZ as the substrate, the activity was increased three-fold after 50 min preincubation at 70°C and 2.4-fold after 10 min preincubation at 80°C. Preincubation of the enzyme in 70°C for 30 min raised the activity four-fold with ABTS as the substrate. Also, l-dopa was used as a substrate. The enzyme was able to oxidize l-dopa with the K M and k cat of 1,493 μM and 194 s−1, respectively. |
| Starting Page | 863 |
| Ending Page | 869 |
| Page Count | 7 |
| File Format | |
| ISSN | 13675435 |
| Journal | Journal of Industrial Microbiology and Biotechnology |
| Volume Number | 37 |
| Issue Number | 8 |
| e-ISSN | 14765535 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2010-05-15 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | Subscribed |
| Subject Keyword | CotA Laccase Expression Microaerobic conditions Biochemical characterization Thermal activation Microbiology Bioinformatics Biotechnology Genetic Engineering Inorganic Chemistry Biochemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology |
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