NDLI: Bacterial growth and substrate degradation by BTX-oxidizing culture in response to salt stress

Content Provider	Springer Nature Link
Author	Lee, Chi Yuan Lin, Ching Hsing
Copyright Year	2005
Abstract	Interactions between microbial growth and substrate degradation are important in determining the performance of trickle-bed bioreactors (TBB), especially when salt is added to reduce biomass formation in order to alleviate media clogging. This study was aimed at quantifying salinity effects on bacterial growth and substrate degradation, and at acquiring kinetic information in order to improve the design and operation of TBB. Experiment works began by cultivating a mixed culture in a chemostat reactor receiving artificial influent containing a mixture of benzene, toluene, and xylene (BTX), followed by using the enrichment culture to degrade the individual BTX substrates under a particular salinity, which ranged 0–50 g l−1 in batch mode. Then, the measured concentrations of biomass and residual substrate versus time were analyzed with the microbial kinetics; moreover, the obtained microbial kinetic constants under various salinities were modeled using noncompetitive inhibition kinetics. For the three substrates the observed bacterial yields appeared to be decreased from 0.51–0.74 to 0.20–0.22 mg mg−1 and the maximum specific rate of substrate utilization, $${\mathop q\limits^ \wedge},$$ declined from 0.25–0.42 to 0.07–0.11 h−1, as the salinity increased from 0 to 50 NaCl g l−1. The NaCl acted as noncompetitive inhibitor, where the modeling inhibitions of the coefficients, K T(S), were 22.7–29.7 g l−1 for substrate degradation and K T(μ), 13.0–19.0 g l−1, for biomass formation. The calculated ratios for the bacterial maintenance rate, m S, to $${\mathop q\limits^ \wedge}$$ further indicated that the percentage energy spent on maintenance increased from 19–24 to 86–91% as salinity level increased from 0 to 50 g l−1. These results revealed that the bacterial growth was more inhibited than substrate degradation by the BTX oxidizers under the tested salinity levels. The findings from this study demonstrate the potential of applying NaCl salt to control excessive biomass formation in biotrickling filters.
Starting Page	37
Ending Page	44
Page Count	8
File Format	PDF
ISSN	13675435
Journal	Journal of Industrial Microbiology and Biotechnology
Volume Number	33
Issue Number	1
e-ISSN	14765535
Language	English
Publisher	Springer-Verlag
Publisher Date	2005-11-12
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Benzene Toluene Xylene Salinity Trickle-bed bioreactor Kinetics Microbial Genetics and Genomics Microbiology Bioinformatics Biochemistry Genetic Engineering Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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