NDLI: Bacterial glyphosate resistance conferred by overexpression of an E. coli membrane efflux transporter

Content Provider	Springer Nature Link
Author	Staub, Jeffrey M. Brand, Leslie Tran, Minhtien Kong, Yifei Rogers, Stephen G.
Copyright Year	2011
Abstract	Glyphosate herbicide-resistant crop plants, introduced commercially in 1994, now represent approximately 85% of the land area devoted to transgenic crops. Herbicide resistance in commercial glyphosate-resistant crops is due to expression of a variant form of a bacterial 5-enolpyruvylshikimate-3-phosphate synthase with a significantly decreased binding affinity for glyphosate at the target site of the enzyme. As a result of widespread and recurrent glyphosate use, often as the only herbicide used for weed management, increasing numbers of weedy species have evolved resistance to glyphosate. Weed resistance is most often due to changes in herbicide translocation patterns, presumed to be through the activity of an as yet unidentified membrane transporter in plants. To provide insight into glyphosate resistance mechanisms and identify a potential glyphosate transporter, we screened Escherichia coli genomic DNA for alternate sources of glyphosate resistance genes. Our search identified a single non-target gene that, when overexpressed in E. coli and Pseudomonas, confers high-level glyphosate resistance. The gene, yhhS, encodes a predicted membrane transporter of the major facilitator superfamily involved in drug efflux. We report here that an alternative mode of glyphosate resistance in E. coli is due to reduced accumulation of glyphosate in cells that overexpress this membrane transporter and discuss the implications for potential alternative resistance mechanisms in other organisms such as plants.
Starting Page	641
Ending Page	647
Page Count	7
File Format	PDF
ISSN	13675435
Journal	Journal of Industrial Microbiology and Biotechnology
Volume Number	39
Issue Number	4
e-ISSN	14765535
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-11-17
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Glyphosate Herbicide Major facilitator superfamily Membrane transporter Inorganic Chemistry Microbiology Bioinformatics Genetic Engineering Biochemistry Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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