NDLI: Optimization of production of caffeine demethylase by Pseudomonas sp. in a bioreactor

Content Provider	Springer Nature Link
Author	Gummadi, Sathyanarayana N. Dash, Swati Sucharita Devarai, Santhosh
Copyright Year	2009
Abstract	The effect of pH, aeration rate, and agitation rate on specific productivity of caffeine demethylase from Pseudomonas sp. was studied in a bioreactor. Maximum specific productivity of caffeine demethylase of 2,214 U g cell dry weight−1 h−1 was obtained at 0.27 vvm, 700 rpm, and pH 7.0. Under these conditions, volumetric oxygen transfer coefficient was 74.2 h−1, indicating that caffeine demethylase production by Pseudomonas sp. was highly oxygen-dependent. Different metabolite formation at different agitation and aeration rates can be used as a strategy for recovery of pharmaceutically important metabolites from caffeine by manipulation of conditions in a bacterial culture. This is the first report on production of high levels of caffeine demethylase in bioreactors.
Starting Page	713
Ending Page	720
Page Count	8
File Format	PDF
ISSN	13675435
Journal	Journal of Industrial Microbiology and Biotechnology
Volume Number	36
Issue Number	5
e-ISSN	14765535
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-02-20
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Aeration rate Agitation rate Bioreactor Caffeine degradation Caffeine demethylase Pseudomonas sp Microbiology Bioinformatics Biotechnology Genetic Engineering Inorganic Chemistry Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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