NDLI: Debittering effect of Actinomucor elegans peptidases on soybean protein hydrolysates

Content Provider	Springer Nature Link
Author	Li, Li Yang, Zuo Yi Yang, Xiao Qun Zhang, Gui He Tang, Shu Ze Chen, Feng
Copyright Year	2007
Abstract	Effects of the enzymes in Actinomucor elegans extract and the enzyme Alcalase 2.4L on debittering the soybean protein hydrolysates were investigated. When the protein was treated only with the latter, a strong bitterness formed; but it decreased if the protein was treated with both the enzymes. The more the enzymes were used, weaker was the bitterness tasted. SDS-PAGE profile and ESI-MS spectrum of the hydrolysates evidenced that the Alcalase could convert the protein into peptides rapidly, while the enzymes in the A. elegans extract were able to further degrade some peptides which were difficult or unable to be hydrolyzed by the Alcalase. Further systematic analysis of the peptidases showed that the Alcalase exhibited a significant endopeptidase activity towards NBZ-Phe-pNA substrate (p < 0.01), whereas many exopeptidases in the A. elegans extract had the carboxypeptidase activity towards N-CBZ-Ile-Leu (p < 0.01). It is concluded that those exopeptidases presented in the A. elegans extract can benefit by decreasing the bitterness of the soybean protein hydroysate. They are also capable of being used with the Alcalase in a single-step enzymatic reaction to prepare the bitterless protein hydrolysate, which may be an efficient application for food industry.
Starting Page	41
Ending Page	47
Page Count	7
File Format	PDF
ISSN	13675435
Journal	Journal of Industrial Microbiology and Biotechnology
Volume Number	35
Issue Number	1
e-ISSN	14765535
Language	English
Publisher	Springer-Verlag
Publisher Date	2007-10-18
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Actinomucor elegans Exopeptidase Endopeptidase Soybean protein hydrolysis Bitterness Microbial Genetics and Genomics Microbiology Bioinformatics Biochemistry Genetic Engineering Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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