NDLI: Alginate production by Azotobacter vinelandii mutants altered in poly-β-hydroxybutyrate and alginate biosynthesis

Content Provider	Springer Nature Link
Copyright Year	2002
Abstract	Mutant AT268 of Azotobacter vinelandii — showing diminished production of poly-β-hydroxybutyrate (PHB) due to a mutation in phbR (the gene coding for the transcriptional activator of the phbBAC biosynthetic operon); mutant CNT26, containing a mutation (muc26) that increases the transcription of gene algD (encoding GDP mannose dehydrogenase, the key enzyme in alginate biosynthesis); and mutant DM, carrying both phbR and muc26 mutations — were characterised in terms of alginate production, broth viscosity, and molecular weight of the alginate. All the mutants evaluated produced 25% less alginate with respect to that produced by the wild type. Unexpectedly and with no apparent relation to the phbR and muc26 mutations, mutant DM exhibited the highest molecular weight ever reported for a bacterial alginate (up to 4×106 Da), with a very low polydispersity index (1.3). Acetyl content in the alginate produced by this strain was low (1.4–2.6%). These characteristics make this mutant a very valuable source for producing alginates with improved properties.
Starting Page	209
Ending Page	213
Page Count	5
File Format	PDF
ISSN	13675435
Journal	Journal of Industrial Microbiology and Biotechnology
Volume Number	29
Issue Number	5
e-ISSN	14765535
Language	English
Publisher	Nature Publishing Group
Publisher Date	2002-11-01
Publisher Place	London
Access Restriction	Subscribed
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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