NDLI: Polyethyleneimine-grafted collagen fiber as a carrier for cell immobilization

Content Provider	Springer Nature Link
Author	Zhu, Deyi Li, Xia Liao, Xuepin Shi, Bi
Copyright Year	2014
Abstract	Collagen fiber (CF), an abundant natural biopolymer, features many favorable properties that make it a potential carrier for cell immobilization. In the present investigation, CF was grafted with polyethyleneimine (PEI) using glutaraldehyde (GA) as the cross-linking agent, resulting in the formation of a novel CF based carrier (CF-PEI). The properties of CF-PEI as a carrier were evaluated by the immobilization of Microbacterium arborescens (CICC 20196), which has glucose isomerase (EC 5.3.1.5) activity. It was found that M. arborescens cells immobilized on CF-PEI exhibited higher glucose isomerization than those using activated carbon or anion exchange resin as the carriers. The Michaelis constant (K m) of the isomerization reaction for the CF-PEI-immobilized M. arborescens cells was 0.528 mol/L, which was slightly higher than that of free cells (0.473 mol/L). In addition, the apparent activation energies (E a) of free and immobilized cells on CF-PEI were almost the same at 60 kJ/mol. In an isomerization reaction of glucose to fructose in a fixed-bed reactor, CF-PEI-immobilized M. arborescens cells showed appreciable activity and operational stability. The corresponding isomerization ratio was as high as 41 % for 20 days, and the half-life was about 40 days.
Starting Page	189
Ending Page	196
Page Count	8
File Format	PDF
ISSN	13675435
Journal	Journal of Industrial Microbiology and Biotechnology
Volume Number	42
Issue Number	2
e-ISSN	14765535
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2014-12-25
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Collagen fiber Polyethyleneimine Cell immobilization Microbacterium arborescens Glucose isomerization Microbiology Biochemistry Inorganic Chemistry Genetic Engineering Biotechnology Bioinformatics
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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