NDLI: Rhf1 gene is involved in the fruiting body production of Cordyceps militaris fungus

Content Provider	Springer Nature Link
Author	Jiang, Keqing Han, Richou
Copyright Year	2015
Abstract	Cordyceps militaris is an important medicinal fungus. Commercialization of this fungus needs to improve the fruiting body production by molecular engineering. An improved Agrobacterium tumefaciens-mediated transformation (ATMT) method was used to select an insertional mutant (g38) which exhibited fast stromatal differentiation and increased yield. The Rhf1 gene encoding filamentation protein was destroyed by a single T-DNA and no Rhf1 transcription was detected in mutant g38. To verify the function of the Rhf1 gene, RNA interference plasmid and overexpression vector of the Rhf1 gene were constructed and transferred to the wild-type JM4 by ATMT. Fast stromatal differentiation and larger fruiting bodies were found in the RNAi-Rhf1 mutants (JM-iRhf1). In the overexpression mutants (JM-OERhf1), neither stromata nor fruiting bodies appeared. The rescued strain (38-OERhf1) showed similar growth characteristics as JM4. These results indicated that the Rhf1 gene was involved in the stromatal differentiation and the shape formation of fruiting bodies.
Starting Page	1183
Ending Page	1196
Page Count	14
File Format	PDF
ISSN	13675435
Journal	Journal of Industrial Microbiology and Biotechnology
Volume Number	42
Issue Number	8
e-ISSN	14765535
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-06-06
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Cordyceps militaris Filamentation protein Stromatal differentiation Fruiting body Microbiology Biochemistry Inorganic Chemistry Genetic Engineering Biotechnology Bioinformatics
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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