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| Content Provider | Springer Nature Link |
|---|---|
| Author | Karbalaei Heidari, Hamid Reza Amoozegar, Mohammad Ali Hajighasemi, Mahbod Ziaee, Abed Ali Ventosa, Antonio |
| Copyright Year | 2008 |
| Abstract | The production of a protease was investigated under conditions of high salinity by the moderately halophilic bacterium Halobacillus karajensis strain MA-2 in a basal medium containing peptone, beef extract, maltose and NaCl when the culture reached the stationary growth phase. Effect of various temperatures, initial pH, salt and different nutrient sources on protease production revealed that the maximum secretion occurred at 34°C, pH 8.0–8.5, and in the presence of gelatin. Replacement of NaCl by various concentrations of sodium nitrate in the basal medium also increased the protease production. The secreted protease was purified 24-fold with 68% recovery by a simple approach including a combination of acetone precipitation and Q-Sepharose ion exchange chromatography. The enzyme revealed a monomeric structure with a relative molecular mass of 36 kDa by running on SDS-PAGE. Maximum caseinolytic activity of the enzyme was observed at 50°C, pH 9.0 and 0.5 M NaCl, although at higher salinities (up to 3 M) activity still remained. The maximum enzyme activity was obtained at a broad pH range of 8.0–10.0, with 55 and 50% activity remaining at pH 6 and 11, respectively. Moreover, the enzyme activity was strongly inhibited by phenylmethylsulfonyl fluoride (PMSF), Pefabloc SC and EDTA; indicating that it probably belongs to the subclass of serine metalloproteases. These findings suggest that the protease secreted by Halobacillus karajensis has a potential for biotechnological applications from its haloalkaline properties point of view. |
| Starting Page | 21 |
| Ending Page | 27 |
| Page Count | 7 |
| File Format | |
| ISSN | 13675435 |
| Journal | Journal of Industrial Microbiology and Biotechnology |
| Volume Number | 36 |
| Issue Number | 1 |
| e-ISSN | 14765535 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2008-09-10 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | Subscribed |
| Subject Keyword | Haloalkaline protease Halobacillus karajensis Moderately halophilic bacteria Protease production Serine metalloproteases Microbiology Bioinformatics Biotechnology Genetic Engineering Inorganic Chemistry Biochemistry |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology |
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