NDLI: Semi-preparative scale purification of enterococcal bacteriocin enterocin EJ97, and evaluation of substrates for its production

Content Provider	Springer Nature Link
Author	López, Rosario Lucas García, Ma. Teresa Abriouel, Hikmate Omar, Nabil Ben Grande, Ma. José Martínez Cañamero, Magdalena Gálvez, Antonio
Copyright Year	2007
Abstract	The influence of substrate composition on the production of enterocin EJ97 and the conditions for semi-preparative bacteriocin recovery have been studied. Final bacteriocin concentrations of 12.5 or 15.6 mg/l were obtained in the commercial media brain heart infusion broth (BHI) and tryptic soya broth, respectively. The bacteriocin was also produced in the complex medium CM (8.75 mg/l), in which the vitamin supplement was essential for production. Some combinations of meat peptone and yeast extract plus either soy peptone or BHI also supported bacteriocin production, at concentrations of 6.25–7.5 mg/l. In cow milk (whole, half-skimmed, and skimmed), the final bacteriocin concentrations obtained ranged from 7.5 to 11.25 mg/l. Highest bacteriocin activity was obtained by using pasteurised milk whey as growth substrate (up to 25 mg/l), suggesting that this bacteriocin can be obtained on a large scale by using this cheap food-grade industrial by-product. Highest bacteriocin titres were always obtained after 8 h of incubation at 37 °C. Semi-preparative concentration and purification of enterocin EJ97 produced in a complex medium was achieved by bulk cation exchange chromatography without previous cell separation, followed by reversed-phase chromatography. This two-step procedure allowed preparation of milligram quantities of purified bacteriocin, which is an improvement compared to purification procedures established for most other bacteriocins (35). The availability of purified enterocin EJ97 will facilitate other studies such as the elucidation of its molecular structure and its interaction with target bacteria.
Starting Page	779
Ending Page	785
Page Count	7
File Format	PDF
ISSN	13675435
Journal	Journal of Industrial Microbiology and Biotechnology
Volume Number	34
Issue Number	12
e-ISSN	14765535
Language	English
Publisher	Springer-Verlag
Publisher Date	2007-09-28
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Bacteriocins Biopreservation Fermentation Microbial Genetics and Genomics Microbiology Bioinformatics Biochemistry Genetic Engineering Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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