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| Content Provider | Springer Nature Link |
|---|---|
| Author | Gong, Guo li Sun, Xin Liu, Xin li Hu, Wei Cao, Wen rui Liu, Hong Liu, Wei feng Li, Yue zhong |
| Copyright Year | 2007 |
| Abstract | The epothilones are highly promising prospective anticancer agents that are produced by the myxobacterium Sorangium cellulosum. We mutated the epothilone producing S. cellulosum strain So0157-2 to improve the production of epothilones. For evaluation in high-throughput of a large number of mutants, we developed a simple microtiter method for primary screening. Using the classical UV-mutation method plus selection pressures, the production capacity was increased about 0.5∼2.5 times the starting strain. The mutants with higher production and different phenotypes were further subjected to recursive protoplast fusions and the fusants products were screened under multi-selection pressure. Furthermore, the production was greatly increased by the genome shuffling. For epothilone B, the production of one fusant was increased about 130 times compared to the starting strain, increasing from 0.8 mg l−1 to 104 mg l−1. |
| Starting Page | 615 |
| Ending Page | 623 |
| Page Count | 9 |
| File Format | |
| ISSN | 13675435 |
| Journal | Journal of Industrial Microbiology and Biotechnology |
| Volume Number | 34 |
| Issue Number | 9 |
| e-ISSN | 14765535 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2007-07-24 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | Subscribed |
| Subject Keyword | Epothilone Sorangium cellulosum Genome shuffling High throughput screening Microbial Genetics and Genomics Microbiology Bioinformatics Biochemistry Genetic Engineering Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology |
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