NDLI: Factors affecting the production of l-xylulose by resting cells of recombinant Escherichia coli

Content Provider	Springer Nature Link
Author	Usvalampi, Anne Kiviharju, Kristiina Leisola, Matti Nyyssölä, Antti
Copyright Year	2009
Abstract	Factors affecting the production of the rare sugar l-xylulose from xylitol using resting cells were investigated. An E. coli BPT228 strain that recombinantly expresses a gene for xylitol dehydrogenase was used in the experiments. The ratio of xylitol to l-xylulose was three times lower in the cytoplasm than in the medium. The effects of pH, temperature, shaking speed, and initial xylitol concentration on l-xylulose production were investigated in shaking flasks using statistical experimental design methods. The highest production rates were found at high shaking speed and at high temperature (over 44°C). The optimal pH for both productivity and conversion was between 7.5 and 8.0, and the optimal xylitol concentration was in the range 250–350 g l−1. A specific productivity of 1.09 ± 0.10 g g−1 h−1 was achieved in a bioreactor. The response surface model based on the data from the shake flask experiments predicted the operation of the process in a bioreactor with reasonable accuracy.
Starting Page	1323
Ending Page	1330
Page Count	8
File Format	PDF
ISSN	13675435
Journal	Journal of Industrial Microbiology and Biotechnology
Volume Number	36
Issue Number	10
e-ISSN	14765535
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-07-15
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	l-Xylulose Xylitol Resting cells Rare sugars Xylitol dehydrogenase Microbiology Bioinformatics Biotechnology Genetic Engineering Inorganic Chemistry Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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