NDLI: Xylitol production from DEO hydrolysate of corn stover by Pichia stipitis YS-30

Content Provider	Springer Nature Link
Author	Rodrigues, Rita C. L. B. Kenealy, William R. Jeffries, Thomas W.
Copyright Year	2011
Abstract	Corn stover that had been treated with vapor-phase diethyl oxalate released a mixture of mono- and oligosaccharides consisting mainly of xylose and glucose. Following overliming and neutralization, a d-xylulokinase mutant of Pichia stipitis, FPL-YS30 (xyl3-∆1), converted the stover hydrolysate into xylitol. This research examined the effects of phosphoric or gluconic acids used for neutralization and urea or ammonium sulfate used as nitrogen sources. Phosphoric acid improved color and removal of phenolic compounds. d-Gluconic acid enhanced cell growth. Ammonium sulfate increased cell yield and maximum specific cell growth rate independently of the acid used for neutralization. The highest xylitol yield (0.61 gxylitol/gxylose) and volumetric productivity (0.18 gxylitol/gxylose l) were obtained in hydrolysate neutralized with phosphoric acid. However, when urea was the nitrogen source the cell yield was less than half of that obtained with ammonium sulfate.
Starting Page	1649
Ending Page	1655
Page Count	7
File Format	PDF
ISSN	13675435
Journal	Journal of Industrial Microbiology and Biotechnology
Volume Number	38
Issue Number	10
e-ISSN	14765535
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-03-22
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Xylitol Pichia stipitis FPL-YS30 Corn stover hemicellulosic hydrolysate Nitrogen source Vapor-phase diethyl oxalate pretreatment Overliming neutralization Biotechnology Inorganic Chemistry Biochemistry Bioinformatics Genetic Engineering Microbiology
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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