NDLI: Medium optimization for keratinase production in hair substrate by a new Bacillus subtilis KD-N2 using response surface methodology

Content Provider	Springer Nature Link
Author	Cai, Chenggang Zheng, Xiaodong
Copyright Year	2009
Abstract	Culture medium for keratinase production from hair substrate by a new Bacillus subtilis strain, KD-N2, was optimized. Effects of culture conditions on keratinase production were tested, and optimal results were obtained with 10% inocula (v/v), 16 g/L hair substrate, an initial pH value of 6.5 and a culture volume of 20 mL. Several carbon sources (sucrose, cornflour) and nitrogen sources (yeast extract, tryptone and peptone) had positive effects on keratinase production, with sucrose giving optimal results. To improve keratinase yield, statistically based experimental designs were applied to optimize the culture medium. Fractional factorial design (FFD) experiments showed that MgSO4 and K2HPO4 were the most significant factors affecting keratinase production. Further central composite design (CCD) experiments indicated that the optimal MgSO4 and K2HPO4 concentrations were 0.91 and 2.38 g/L, respectively. Using an optimized fermentation medium (g/L: NaCl 1.0, CaCl2 0.05, KH2PO4 0.7, sucrose 3, MgSO4 0.91, K2HPO4 2.38), keratinase activity increased to 125 U/mL, an approximate 1.7-fold increase over the previous activity (75 U/mL). Human hair was degraded during the submerged cultivation.
Starting Page	875
Ending Page	883
Page Count	9
File Format	PDF
ISSN	13675435
Journal	Journal of Industrial Microbiology and Biotechnology
Volume Number	36
Issue Number	7
e-ISSN	14765535
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-04-07
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Human hair Keratinase Optimization Response surface methodology Microbiology Bioinformatics Biotechnology Genetic Engineering Inorganic Chemistry Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Enhanced pullulan production in a biofilm reactor by using response surface methodology

Production of biosurfactant by Bacillus subtilis LB5a on a pilot scale using cassava wastewater as substrate

Optimization of fermentation parameters in phage production using response surface methodology

Optimization of medium composition for actinomycin X2 production by Streptomyces spp JAU4234 using response surface methodology

Efficient extracellular expression of Bacillus deramificans pullulanase in Brevibacillus choshinensis

Evaluation and optimization of ethanol production from carob pod extract by Zymomonas mobilis using response surface methodology

Production and optimization of cellulase-free, alkali-stable xylanase by Bacillus pumilus SV-85S in submerged fermentation

Substrate specificity characterization of a thermostable keratinase from Pseudomonas aeruginosa KS-1

Chemostat-induced uneven division of Bacillus subtilis

Medium optimization for keratinase production in hair substrate by a new Bacillus subtilis KD-N2 using response surface methodology

Similar Documents

Enhanced pullulan production in a biofilm reactor by using response surface methodology

Production of biosurfactant by Bacillus subtilis LB5a on a pilot scale using cassava wastewater as substrate

Optimization of fermentation parameters in phage production using response surface methodology

Optimization of medium composition for actinomycin X2 production by Streptomyces spp JAU4234 using response surface methodology

Efficient extracellular expression of Bacillus deramificans pullulanase in Brevibacillus choshinensis

Evaluation and optimization of ethanol production from carob pod extract by Zymomonas mobilis using response surface methodology

Production and optimization of cellulase-free, alkali-stable xylanase by Bacillus pumilus SV-85S in submerged fermentation

Substrate specificity characterization of a thermostable keratinase from Pseudomonas aeruginosa KS-1

Chemostat-induced uneven division of Bacillus subtilis

Medium optimization for keratinase production in hair substrate by a new Bacillus subtilis KD-N2 using response surface methodology