NDLI: Methods for the genetic manipulation of Nonomuraea sp. ATCC 39727

Content Provider	Springer Nature Link
Author	Marcone, Giorgia Letizia Foulston, Lucy Binda, Elisa Marinelli, Flavia Bibb, Mervyn Beltrametti, Fabrizio
Copyright Year	2010
Abstract	Nonomuraea sp. ATCC 39727 belongs to the Streptosporangiaceae family of filamentous actinomycetes. This microorganism produces the teicoplanin-like glycopeptide A40926, which is the starting material for the synthesis of the second-generation glycopeptide dalbavancin. Notwithstanding the strain’s pharmaceutical relevance, the lack or poor efficiency of genetic tools to manipulate Nonomuraea sp. ATCC 39727 has hampered strain and product improvement. Here we report the development of gene transfer systems based on protoplast transformation and intergeneric conjugation from Escherichia coli. Efficiency of transformation and conjugation, followed by site specific or homologous recombination with the Nonomuraea sp. genome, were determined using the integrative plasmid pSET152 (5.7 kb), and the Supercos1 derivative cosmid A40ΔY (30 kb). To our knowledge, this is the first report of the transformation of protoplasts of Nonomuraea sp. ATCC 39727, even though the improved procedure for intergeneric conjugation makes it the method of choice for introducing large segments of DNA into Nonomuraea sp. ATCC 39727.
Starting Page	1097
Ending Page	1103
Page Count	7
File Format	PDF
ISSN	13675435
Journal	Journal of Industrial Microbiology and Biotechnology
Volume Number	37
Issue Number	10
e-ISSN	14765535
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-08-18
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Actinomycetes Antibiotics Nonomuraea Conjugation Protoplasts Microbiology Bioinformatics Biotechnology Genetic Engineering Inorganic Chemistry Biochemistry
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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