NDLI: High-yield production of the VP1 structural protein epitope from serotype O foot-and-mouth disease virus in Escherichia coli

Content Provider	Springer Nature Link
Author	Jung, Joon Goo Lee, Yong Jae Velmurugan, Natarajan Ko, Young Joon Lee, Hyang Sim Jeong, Ki Jun
Copyright Year	2013
Abstract	For effective control of foot-and-mouth disease (FMD), the development of rapid diagnostic systems and vaccines are required against its etiological agent, FMD virus (FMDV). To accomplish this, efficient large-scale expression of the FMDV VP1 protein, with high solubility, needs to be optimized. We attempted to produce high levels of a serotype O FMDV VP1 epitope in Escherichia coli. We identified the subtype-independent serotype O FMDV VP1 epitope sequence and used it to construct a glutathione S-transferase (GST) fusion protein. For efficient production of the FMDV VP1 epitope fused to GST (VP1e–GST), four E. coli strains and three temperatures were examined. The conditions yielding the greatest level of VP1e–GST with highest solubility were achieved with E. coli BL21(DE3) at 25 °C. For high-level production, fed-batch cultures were conducted in 5-l bioreactors. When cells were induced at a high density and complex feeding solutions were supplied, approximately 11 g of VP1e–GST was obtained from a 2.9-l culture. Following purification, the VP1 epitope was used to immunize rabbits, and we confirmed that it induced an immune response.
Starting Page	705
Ending Page	713
Page Count	9
File Format	PDF
ISSN	13675435
Journal	Journal of Industrial Microbiology and Biotechnology
Volume Number	40
Issue Number	7
e-ISSN	14765535
Language	English
Publisher	Springer-Verlag
Publisher Date	2013-04-26
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Escherichia coli Fed-batch cultivation FMDV VP1 epitope Biochemistry Inorganic Chemistry Genetic Engineering Biotechnology Bioinformatics Microbiology
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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