NDLI: Expression of plectasin in Bacillus subtilis using SUMO technology by a maltose-inducible vector

Content Provider	Springer Nature Link
Author	Zhang, Licong Li, Xiaodan Wei, Dandan Wang, Jue Shan, Anshan Li, Zhongyu
Copyright Year	2015
Abstract	Plectasin, the first fungus defensin, is especially efficient against Gram-positive bacteria. To explore an effective approach for expressing plectasin in Bacillus subtilis, the sequence encoding plectasin fused with the small ubiquitin-like modifier (SUMO) gene, the 6 × His gene and the signal peptide of SacB were cloned into an E. coli–B. subtilis shuttle vector pGJ148 in which the maltose utilization operon promoter Pglv directed the expression. The fusion protein successfully secreted in culture and approximately, 41 mg of the recombinant fusion protein SUMO-plectasin was purified per liter of culture supernatant. After purification by Ni-NTA resin column and digestion by SUMO protease, 5.5 mg of plectasin with a purity of 94 % was obtained from 1 L fermentation culture. Recombinant plectasin was found inhibition activity against S. pneumoniae, S. aureus and S. epidermidis. These results indicate that the maltose-induced expression system may be a safe and efficient way for the large-scale production of soluble peptides in B. subtilis.
Starting Page	1369
Ending Page	1376
Page Count	8
File Format	PDF
ISSN	13675435
Journal	Journal of Industrial Microbiology and Biotechnology
Volume Number	42
Issue Number	10
e-ISSN	14765535
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-08-25
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Antimicrobial peptide Plectasin Bacillus subtilis Maltose-inducible promoter SUMO Microbiology Biochemistry Inorganic Chemistry Genetic Engineering Biotechnology Bioinformatics
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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