NDLI: Heterologous production of daptomycin in Streptomyces lividans

Content Provider	Springer Nature Link
Author	Penn, Julia Li, Xiang Whiting, Andrew Latif, Mohammed Gibson, Trevor Silva, Christopher J. Brian, Paul Davies, Julian Miao, Vivian Wrigley, Stephen K. Baltz, Richard H.
Copyright Year	2005
Abstract	Daptomycin and the A21978C antibiotic complex are lipopeptides produced by Streptomyces roseosporus and also in recombinant Streptomyces lividans TK23 and TK64 strains, when a 128 kbp region of cloned S. roseosporus DNA containing the daptomycin gene cluster is inserted site-specifically in the ϕC31 attB site. A21978C fermentation yields were initially much lower in S. lividans than in S. roseosporus, and detection was complicated by the production of host metabolites. However A21978C production in S. lividans was improved by deletion of genes encoding the production of actinorhodin and by medium optimization to control the chemical form of the calcium dependent antibiotic (CDA). This latter compound has not previously been chemically characterized as a S. lividans product. Adding phosphate to a defined fermentation medium resulted in formation of only the phosphorylated forms of CDA, which were well separated from A21978C on chromatographic analysis. Adjusting the level of phosphate in the medium led to an improvement in A21978C yield from 20 to 55 mg/l.
Starting Page	121
Ending Page	128
Page Count	8
File Format	PDF
ISSN	13675435
Journal	Journal of Industrial Microbiology and Biotechnology
Volume Number	33
Issue Number	2
e-ISSN	14765535
Language	English
Publisher	Springer-Verlag
Publisher Date	2005-11-01
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Daptomycin A21978C Calcium-dependent antibiotic (CDA) Actinorhodin Streptomyces lividans Microbial Genetics and Genomics Microbiology Bioinformatics Biochemistry Genetic Engineering Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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