NDLI: A versatile method for preparation of hydrated microbial–latex biocatalytic coatings for gas absorption and gas evolution

Content Provider	Springer Nature Link
Author	Gosse, Jimmy L. Chinn, Mari S. Grunden, Amy M. Bernal, Oscar I. Jenkins, Jessica S. Yeager, Chris Kosourov, Sergey Seibert, Michael Flickinger, Michael C.
Copyright Year	2012
Abstract	We describe a latex wet coalescence method for gas-phase immobilization of microorganisms on paper which does not require drying for adhesion. This method reduces drying stresses to the microbes. It is applicable for microorganisms that do not tolerate desiccation stress during latex drying even in the presence of carbohydrates. Small surface area, 10–65 μm thick coatings were generated on chromatography paper strips and placed in the head-space of vertical sealed tubes containing liquid to hydrate the paper. These gas-phase microbial coatings hydrated by liquid in the paper pore space demonstrated absorption or evolution of H2, CO, CO2 or O2. The microbial products produced, ethanol and acetate, diffuse into the hydrated paper pores and accumulate in the liquid at the bottom of the tube. The paper provides hydration to the back side of the coating and also separates the biocatalyst from the products. Coating reactivity was demonstrated for Chlamydomonas reinhardtii CC124, which consumed CO2 and produced 10.2 ± 0.2 mmol O2 m−2 h−1, Rhodopseudomonas palustris CGA009, which consumed acetate and produced 0.47 ± 0.04 mmol H2 m−2 h−1, Clostridium ljungdahlii OTA1, which consumed 6 mmol CO m−2 h−1, and Synechococcus sp. PCC7002, which consumed CO2 and produced 5.00 ± 0.25 mmol O2 m−2 h−1. Coating thickness and microstructure were related to microbe size as determined by digital micrometry, profilometry, and confocal microscopy. The immobilization of different microorganisms in thin adhesive films in the gas phase demonstrates the utility of this method for evaluating genetically optimized microorganisms for gas absorption and gas evolution.
Starting Page	1269
Ending Page	1278
Page Count	10
File Format	PDF
ISSN	13675435
Journal	Journal of Industrial Microbiology and Biotechnology
Volume Number	39
Issue Number	9
e-ISSN	14765535
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-05-17
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Latex coating immobilization on chromatography paper Chlamydomonas Rhodopseudomonas Clostridium Synechococcus Inorganic Chemistry Biochemistry Bioinformatics Genetic Engineering Microbiology Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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