NDLI: Development of engineered Escherichia coli whole-cell biocatalysts for high-level conversion of l-lysine into cadaverine

Content Provider	Springer Nature Link
Author	Oh, Young Hoon Kang, Kyoung Hee Kwon, Mi Jeong Choi, Jae Woo Joo, Jeong Chan Lee, Seung Hwan Yang, Yung Hun Song, Bong Keun Kim, Il Kwon Yoon, Ki Hoon Park, Kyungmoon Park, Si Jae
Copyright Year	2015
Abstract	A whole-cell biocatalytic system for the production of cadaverine from l-lysine has been developed. Among the investigated lysine decarboxylases from different microorganisms, Escherichia coli LdcC showed the best performance on cadaverine synthesis when E. coli XL1-Blue was used as the host strain. Six different strains of E. coli expressing E. coli LdcC were investigated and recombinant E. coli XL1-Blue, BL21(DE3) and W were chosen for further investigation since they showed higher conversion yield of lysine into cadaverine. The effects of substrate pH, substrate concentrations, buffering conditions, and biocatalyst concentrations have been investigated. Finally, recombinant E. coli XL1-Blue concentrated to an OD600 of 50, converted 192.6 g/L (1317 mM) of crude lysine solution, obtained from an actual lysine manufacturing process, to 133.7 g/L (1308 mM) of cadaverine with a molar yield of 99.90 %. The whole-cell biocatalytic system described herein is expected to be applicable to the development of industrial bionylon production process.
Starting Page	1481
Ending Page	1491
Page Count	11
File Format	PDF
ISSN	13675435
Journal	Journal of Industrial Microbiology and Biotechnology
Volume Number	42
Issue Number	11
e-ISSN	14765535
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2015-09-12
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Cadaverine l-Lysine Whole-cell conversion Bio-catalyst Recombinant E. coli Microbiology Biochemistry Inorganic Chemistry Genetic Engineering Biotechnology Bioinformatics
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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