NDLI: A comprehensive analysis of the effects of the main component enzymes of cellulase derived from Trichoderma reesei on biomass saccharification

Content Provider	Springer Nature Link
Author	Kawai, Tetsushi Nakazawa, Hikaru Ida, riko Okada, Hirofumi Ogasawara, Wataru Morikawa, Yasushi Kobayashi, Yoshiri
Copyright Year	2013
Abstract	The aim of this study was a comprehensive analysis of the effects of the component enzymes of cellulase derived from Trichoderma reesei strain PC-3-7 on biomass saccharification. We used cellulases with deleted CBH I, CBH II, or EG I, which contain all other component enzymes, for saccharification of differently pretreated biomasses of rice straw, Erianthus, eucalyptus, and Japanese cedar. We found that CBH I was the most effective in saccharification of all pretreated cellulosic biomasses, although the effect was weaker in saccharification of sulfuric acid- and hydrothermally pretreated rice straw than of others; CBH II was more effective for rice straw than for eucalyptus, and was the most effective at the early stages of biomass degradation; EG I had little effect on pretreated biomasses, in particular, it had no effect on steam-exploded Japanese cedar. Thus, the effects of the main component enzymes depend on the biomass source and pretreatment. These findings will likely help to improve cellulase for industrial use.
Starting Page	805
Ending Page	810
Page Count	6
File Format	PDF
ISSN	13675435
Journal	Journal of Industrial Microbiology and Biotechnology
Volume Number	40
Issue Number	8
e-ISSN	14765535
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2013-06-04
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Cellulase Trichoderma reesei Component enzymes Bioconversion Enzymatic saccharification Microbiology Biochemistry Inorganic Chemistry Genetic Engineering Biotechnology Bioinformatics
Content Type	Text
Resource Type	Article
Subject	Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology

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