NDLI: Highly sensitive single-fibril erosion assay demonstrates mechanochemical switch in native collagen fibrils

Content Provider	Springer Nature Link
Author	Flynn, Brendan P. Tilburey, Graham E. Ruberti, Jeffrey W.
Copyright Year	2012
Abstract	It has been established that the enzyme susceptibility of collagen, the predominant load-bearing protein in vertebrates, is altered by applied tension. However, whether tensile force increases or decreases the susceptibility to enzyme is a matter of contention. It is critical to establish a definitive understanding of the direction and magnitude of the force versus catalysis rate (k C ) relationship if we are to properly interpret connective tissue development, growth, remodeling, repair, and degeneration. In this investigation, we examine collagen/enzyme mechanochemistry at the smallest scale structurally relevant to connective tissue: the native collagen fibril. A single-fibril mechanochemical erosion assay with nN force resolution was developed which permits detection of the loss of a few layers of monomer from the fibril surface. Native type I fibrils (bovine) held at three levels of tension were exposed to Clostridium histolyticum collagenase A. Fibrils held at zero-load failed rapidly and consistently (20 min) while fibrils at 1.8 pN/monomer failed more slowly (35–55 min). Strikingly, fibrils at 23.9 pN/monomer did not exhibit detectable degradation. The extracted force versus k C data were combined with previous single-molecule results to produce a “master curve” which suggests that collagen degradation is governed by an extremely sensitive mechanochemical switch.
Starting Page	291
Ending Page	300
Page Count	10
File Format	PDF
ISSN	16177959
Journal	Biomechanics and Modeling in Mechanobiology
Volume Number	12
Issue Number	2
e-ISSN	16177940
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-05-15
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Collagen ECM (extracellular matrix) Mechanical test Enzymatic degradation Theoretical and Applied Mechanics Biomedical Engineering Biophysics and Biological Physics
Content Type	Text
Resource Type	Article
Subject	Mechanical Engineering Modeling and Simulation Biotechnology

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