NDLI: Mechanical loading of mouse caudal vertebrae increases trabecular and cortical bone mass-dependence on dose and genotype

Content Provider	Springer Nature Link
Author	Webster, Duncan Wasserman, Elad Ehrbar, Martin Weber, Franz Bab, Itai Müller, Ralph
Copyright Year	2010
Abstract	Most in vivo studies addressing the skeletal responses of mice to mechanical loading have targeted cortical bone. To investigate trabecular bone responses also we have developed a caudal vertebral axial compression device (CVAD) that transmits mechanical loads to compress the fifth caudal vertebra via stainless steel pins inserted into the forth and sixth caudal vertebral bodies. Here, we used the CVAD in C57BL/6 (B6) and C3H/Hej (C3H) female mice (15 weeks of age) to investigate whether the effect of regular bouts of mechanical stimulation on bone modeling and bone mass was dependent on dose and genotype. A combined micro-computed tomographic and dynamic histomorphometric analysis was carried out at the end of a 4-week loading regimen (3,000 cycles, 10 Hz, 3 x week) for load amplitudes of 0N, 2N, 4N and 8N. Significant increases in trabecular bone mass of 9 and 21% for loads of 4N and 8N, respectively, were observed in B6 mice. A significant increase of 10% in trabecular bone mass occurred for a load of 8N in the C3H strain. For other loads, no significant increases were detected. Both mouse strains exhibited substantial increases in trabecular bone formation rates for all loads, B6: 111% (2N), 86% (4N), 164% (8N), C3H: 41% (2N), 38% (4N), 141% (8N). Significant decreases in osteoclast number of 146 and 93% for a load of 8N were detected in B6 and C3H mice, respectively. These findings demonstrate that the effect of loading on the structural and functional parameters of bone is dose and genotype dependent. The caudal vertebral loading model established here is proposed for further studies addressing the molecular processes involved in the skeletal responses to mechanical stimuli.
Starting Page	737
Ending Page	747
Page Count	11
File Format	PDF
ISSN	16177959
Journal	Biomechanics and Modeling in Mechanobiology
Volume Number	9
Issue Number	6
e-ISSN	16177940
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-03-30
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Mechanical loading Trabecular bone Cortical bone Bone adaptation Bone morphometry Biophysics and Biological Physics Biomedical Engineering Theoretical and Applied Mechanics
Content Type	Text
Resource Type	Article
Subject	Mechanical Engineering Modeling and Simulation Biotechnology

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