NDLI: Geographic differentiation of Cobitis shikokuensis inferred from mtDNA RFLP analysis

Content Provider	Springer Nature Link
Author	Shimizu, Takaaki
Copyright Year	2008
Abstract	Mitochondrial DNA divergence among populations of the Japanese spinous loach Cobitis shikokuensis, endemic to Shikoku Island, was investigated by restricted fragment length polymorphism analysis. A total of 68 restriction sites on DNA fragments from the cytochrome b to D-loop regions and from the 12S rRNA to 16S rRNA regions, amplified by PCR, were analyzed. A total of 12 haplotypes (plus 6 in outgroups) were detected in 268 specimens collected from 19 localities in seven rivers (and 41 specimens from four localities in three rivers in outgroups). Three of the seven river populations of C. shikokuensis were shown to have unique haplotypes, and four of the seven river populations were monomorphic. The nested structure of the haplotype network for populations of C. shikokuensis exhibited two large clades corresponding to (1) populations from the Shimanto River and its neighbors and (2) two genetically divergent populations in the Shigenobu and Iwamatsu Rivers. The population from the Shimanto River, the largest river inhabited by C. shikokuensis, maintains great haplotype diversity as well as the allozyme diversity previously reported. On the other hand, populations from the Hiji River, the second largest river, which exhibited the highest allozyme diversity, were monomorphic in their mtDNA. The nested clade analysis (NCA) revealed that past fragmentation between the above two clades could occur in the initial distribution process of C. shikokuensis. The large genetic divergence of two river populations from the Shigenobu and Iwamatsu Rivers was inferred to be caused by a process of long distance colonization and fragmentation. MtDNA introgression into the Hiji River population from southern river populations was suggested. Taking genetic divergence into consideration, each river population of C. shikokuensis should be conserved separately as like a distinct species, and conservation programs for the small populations showing less genetic variability should be invoked as soon as possible.
Starting Page	101
Ending Page	111
Page Count	11
File Format	PDF
ISSN	13418998
Journal	Ichthyological Research
Volume Number	55
Issue Number	2
e-ISSN	16163915
Language	English
Publisher	Springer Japan
Publisher Date	2008-02-05
Publisher Place	Japan
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cobitidae PCR-RFLP Nested clade analysis (NCA) Conservation Evolutionarily significant unit (ESU) Freshwater & Marine Ecology Animal Systematics/Taxonomy/Biogeography Ecology Zoology
Content Type	Text
Resource Type	Article
Subject	Ecology, Evolution, Behavior and Systematics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

A new loach, Cobitis shikokuensis (Teleostei: Cobitidae), from Shikoku Island, Japan

Habitat factors affecting the distribution and abundance of the spinous loach Cobitis shikokuensis in southwestern Japan

Threatened fishes of the world: Cobitis narentana Karaman, 1928 (Cobitidae)

Threatened fishes of the world: Cobitis linea (Heckel, 1849) (Cobitidae)

Rapid and cost-effective molecular identification of the three mackerel species of the genus Rastrelliger (Perciformes: Scombridae) using PCR-RFLP analysis

Genetic relationships among the Japanese and Korean striated spined loach complex (Cobitidae: Cobitis) and their phylogenetic positions

Threatened Fishes of the World, Cobitis puncticulata (Erk’akan, Atalay-Ekmekçi & Nalbant, 1998) (Cobitidae)

Cobitis sakahoko, a new species of spined loach (Cypriniformes: Cobitidae) from southern Kyushu Island, Japan

Significant genetic differentiation between native and introduced silver carp (Hypophthalmichthys molitrix) inferred from mtDNA analysis

Geographic differentiation of Cobitis shikokuensis inferred from mtDNA RFLP analysis

Similar Documents

A new loach, Cobitis shikokuensis (Teleostei: Cobitidae), from Shikoku Island, Japan

Habitat factors affecting the distribution and abundance of the spinous loach Cobitis shikokuensis in southwestern Japan

Threatened fishes of the world: Cobitis narentana Karaman, 1928 (Cobitidae)

Threatened fishes of the world: Cobitis linea (Heckel, 1849) (Cobitidae)

Rapid and cost-effective molecular identification of the three mackerel species of the genus Rastrelliger (Perciformes: Scombridae) using PCR-RFLP analysis

Genetic relationships among the Japanese and Korean striated spined loach complex (Cobitidae: Cobitis) and their phylogenetic positions

Threatened Fishes of the World, Cobitis puncticulata (Erk’akan, Atalay-Ekmekçi & Nalbant, 1998) (Cobitidae)

Cobitis sakahoko, a new species of spined loach (Cypriniformes: Cobitidae) from southern Kyushu Island, Japan

Significant genetic differentiation between native and introduced silver carp (Hypophthalmichthys molitrix) inferred from mtDNA analysis

Geographic differentiation of Cobitis shikokuensis inferred from mtDNA RFLP analysis