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| Content Provider | Springer Nature Link |
|---|---|
| Author | Hong, Yantao Chen, Longfei Du, Li pu Su, Zhenqi Wang, Jinfen Ye, Xingguo Qi, Lin Zhang, Zengyan |
| Copyright Year | 2014 |
| Abstract | Bread wheat (Triticum aestivum L.) is a major staple crop in the world. Grain weight is a major factor of grain yield in wheat, and the identification of candidate genes associated with grain weight is very important for high-yield breeding of wheat. TaGW2 is an orthologous gene of rice OsGW2 that negatively regulates the grain width and weight in rice. There are three TaGW2 homoeologs in bread wheat, TaGW2A, TaGW2B, and TaGW2D. In this study, a specific TaGW2-RNA interference (RNAi) cassette was constructed and transformed into a Chinese bread wheat variety ‘Shi 4185’ with small grain. The transcript levels of TaGW2A, TaGW2B, and TaGW2D were simultaneously downregulated in TaGW2-RNAi transgenic wheat lines. Compared with the controls, TaGW2-underexpressing transgenic lines displayed significantly increases in the grain width and weight, suggesting that TaGW2 negatively regulated the grain width and weight in bread wheat. Further transcript analysis showed that in different bread wheat accessions, the transcript abundance of TaGW2A was negatively associated with the grain width. |
| Starting Page | 341 |
| Ending Page | 349 |
| Page Count | 9 |
| File Format | |
| ISSN | 1438793X |
| Journal | Functional & Integrative Genomics |
| Volume Number | 14 |
| Issue Number | 2 |
| e-ISSN | 14387948 |
| Language | English |
| Publisher | Springer Berlin Heidelberg |
| Publisher Date | 2014-06-03 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Bread wheat (Triticum aestivum L.) Functional analysis Grain width RNA interference TaGW2 Cell Biology Plant Genetics & Genomics Microbial Genetics and Genomics Biochemistry Bioinformatics Animal Genetics and Genomics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Medicine |
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