NDLI: Novel miRNAs in the control of arsenite levels in rice

Content Provider	Springer Nature Link
Author	Liu, Qingpo
Copyright Year	2012
Abstract	In a species, the miRNA repertoire comprises many lineage- or species-specific miRNAs. Using deep sequencing, the whole transcriptome of seedling roots was studied and a total of 18 novel miRNAs in indica rice Minghui 86 were identified. Among these novel miRNAs six were up-regulated and two were down-regulated under arsenite stress. Quantitative real-time RT-PCR analysis revealed that miR6254 was predominantly expressed in roots relative to other tissues, whereas miR6250 and miR169i-3p were constitutively expressed in all tissues examined, with high abundance in roots (miR6250) or leaves (miR169i-3p). The miR6250 and miR169i-3p miRNAs also exhibited distinct expression patterns in rice cultivars Minghui 86 and Nipponbare at different time points after arsenite treatment. The predicted targets for these miRNAs included some protein kinases, DNA, or ATP-binding proteins. Besides arsenite, the expression of targets of miR6250 and miR6254 was also up- or down-regulated in response to abiotic environmental stresses, indicative of their involvement in regulation of plant adaptation. Three types of cis-elements involved in hormone, light, and stress response were found to occur frequently in the promoter regions. Interestingly, miR6254 was originally characterized to be an exonic miRNA located in the exon of AK101391, supporting the notion that miRNAs may also originate from some exons in plants.
Starting Page	649
Ending Page	658
Page Count	10
File Format	PDF
ISSN	1438793X
Journal	Functional & Integrative Genomics
Volume Number	12
Issue Number	4
e-ISSN	14387948
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-05-15
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Rice Arsenite Novel miRNA Expression Exonic origination Deep sequencing Microbial Genetics and Genomics Bioinformatics Plant Genetics & Genomics Animal Genetics and Genomics Cell Biology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine

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