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| Content Provider | Springer Nature Link |
|---|---|
| Author | Pandey, Sarita Shrivastava, A. K. Singh, V. K. Rai, R. Singh, P. K. Rai, S. Rai, L. C. |
| Copyright Year | 2012 |
| Abstract | In silico analysis followed by experimental validation leads us to propose that the predicted protein All0195 of Anabaena sp. PCC7120 showing enhanced expression under sodium arsenate (Na2HAsO4) stress belongs to the thioredoxin superfamily with structural similarity to bacterial arsenate reductase. The All0195 protein demonstrated C-X-TC-X-K, NTSG-X2-YR, and D-X2-L-X-KRP as functional motifs that show similarity to seven known bacterial arsenate reductase family protein homologs with Cys, Arg, and Pro as conserved residues. In view of physicochemical properties, such as aliphatic index, ratio of Glu + Lys to Gln + His, and secondary structure, it was evident that All0195 was also a thermostable protein. The predicted three-dimensional structure on molecular docking with arsenate oxyanion ( $$ HAsO_4^{- 2 } $$ ) revealed its interaction with conserved Cys residue as also known for other bacterial arsenate reductase. In silico derived properties were experimentally attested by cloning and heterologous expression of all0195. Furthermore, this protein functionally complemented the arsenate reductase-deficient sodium arsenate-hypersensitive phenotype of Escherichia coli strainWC3110 (ΔarsC) and depicted arsenate reductase activity on purification. In view of the above properties, All0195 appears to be a new arsenate reductase involved in arsenic detoxification in Anabaena sp. PCC7120. |
| Starting Page | 43 |
| Ending Page | 55 |
| Page Count | 13 |
| File Format | |
| ISSN | 1438793X |
| Journal | Functional & Integrative Genomics |
| Volume Number | 13 |
| Issue Number | 1 |
| e-ISSN | 14387948 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2012-10-21 |
| Publisher Place | Berlin, Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Arsenic Arsenate reductase Homology modeling Complementation Cell Biology Plant Genetics & Genomics Microbial Genetics and Genomics Biochemistry Bioinformatics Animal Genetics and Genomics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Medicine |
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