NDLI: The α-gliadin genes from Brachypodium distachyon L. provide evidence for a significant gap in the current genome assembly

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The α-gliadin genes from Brachypodium distachyon L. provide evidence for a significant gap in the current genome assembly

Content Provider	Springer Nature Link
Author	Chen, G. X. Lv, D. W. Li, W. D. Subburaj, S. Yu, Z. T. Wang, Y. J. Li, X. H. Wang, K. Ye, X. G. Ma, Wujun Yan, Y. M.
Copyright Year	2013
Abstract	Brachypodium distachyon, is a new model plant for most cereal crops while gliadin is a class of wheat storage proteins related with wheat quality attributes. In the published B. distachyon genome sequence databases, no gliadin gene is found. In the current study, a number of gliadin genes in B. distachyon were isolated, which is contradictory to the results of genome sequencing projects. In our study, the B. distachyon seeds were found to have no gliadin protein expression by gel electrophoresis, reversed-phase high-performance liquid chromatography and Western blotting analysis. However, Southern blotting revealed a presence of more than ten copies of α-gliadin coding genes in B. distachyon. By means of AS-PCR amplification, four novel full-ORF α-gliadin genes, and 26 pseudogenes with at least one stop codon as well as their promoter regions were cloned and sequenced from different Brachypodium accessions. Sequence analysis revealed a few of single-nucleotide polymorphisms among these genes. Most pseudogenes were resulted from a C to T change, leading to the generation of TAG or TAA in-frame stop codon. To compare both the full-ORFs and the pseudogenes among Triticum and Triticum-related species, their structural characteristics were analyzed. Based on the four T cell stimulatory toxic epitopes and two ployglutamine domains, Aegilops, Triticum, and Brachypodium species were found to be more closely related. The phylogenetic analysis further revealed that B. distachyon was more closely related to Aegilops tauschii, Aegilops umbellulata, and the A or D genome of Triticum aestivum. The α-gliadin genes were able to express successfully in E. coli using the functional T7 promoter. The relative and absolute quantification of the transcripts of α-gliadin genes in wheat was much higher than that in B. distachyon. The abundant pseudogenes may affect the transcriptional and/or posttranscriptional level of the α-gliadin in B. distachyon.
Starting Page	149
Ending Page	160
Page Count	12
File Format	PDF
ISSN	1438793X
Journal	Functional & Integrative Genomics
Volume Number	14
Issue Number	1
e-ISSN	14387948
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2013-12-07
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Brachypodium distachyon Triticum-related species α-gliadins Toxic epitopes Phylogeny and evolution Cell Biology Plant Genetics & Genomics Microbial Genetics and Genomics Biochemistry Bioinformatics Animal Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine

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