NDLI: ES cell neural differentiation reveals a substantial number of novel ESTs

Content Provider	Springer Nature Link
Author	Bain, G. Mansergh, F.C. Wride, M.A. Hance, J.E. Isogawa, A. Rancourt, S.L. Ray, W.J. Yoshimura, Y. Tsuzuki, T. Gottlieb, D.I. Rancourt, D.E.
Copyright Year	2000
Abstract	We have used a method for synchronously differentiating murine embryonic stem (ES) cells into functional neurons and glia in culture. Using subtractive hybridization we isolated approximately 1200 cDNA clones from ES cell cultures at the neural precursor stage of neural differentiation. Pilot studies indicated that this library is a good source of novel neuro-embryonic cDNA clones. We therefore screened the entire library by single-pass sequencing. Characterization of 604 non-redundant cDNA clones by BLAST revealed 96 novel expressed sequence tags (ESTs) and an additional 197 matching uncharacterized ESTs or genomic clones derived from genome sequencing projects. With the exception of a handful of genes, whose functions are still unclear, most of the 311 known genes identified in this screen are expressed in embryonic development and/or the nervous system. At least 80 of these genes are implicated in disorders of differentiation, neural development and/or neural function. This study provides an initial snapshot of gene expression during early neural differentiation of ES cell cultures. Given the recent identification of human ES cells, further characterization of these novel and uncharacterized ESTs has the potential to identify genes that may be important in nervous system development, physiology and disease.
Starting Page	127
Ending Page	139
Page Count	13
File Format	PDF
ISSN	1438793X
Journal	Functional & Integrative Genomics
Volume Number	1
Issue Number	2
e-ISSN	14387948
Language	English
Publisher	Springer-Verlag
Publisher Date	2000-05-16
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cell Biology Plant Genetics & Genomics Microbial Genetics and Genomics Biochemistry Bioinformatics Animal Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Genetics Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Erratum to ES cell neural differentiation reveals a substantial number of novel ESTs

Analysis of SSH library of rice variety Aganni reveals candidate gall midge resistance genes

A look through cereal genomics

Eugenol synthase genes in floral scent variation in Gymnadenia species

Genomic sequencing reveals gene content, genomic organization, and recombination relationships in barley

Yeast and drug discovery

Transposon tagging in maize

Social responsibility in human genetics – a survey

A genome-wide survey reveals a deletion polymorphism associated with resistance to gastrointestinal nematodes in Angus cattle

ES cell neural differentiation reveals a substantial number of novel ESTs

Similar Documents

Erratum to ES cell neural differentiation reveals a substantial number of novel ESTs

Analysis of SSH library of rice variety Aganni reveals candidate gall midge resistance genes

A look through cereal genomics

Eugenol synthase genes in floral scent variation in Gymnadenia species

Genomic sequencing reveals gene content, genomic organization, and recombination relationships in barley

Yeast and drug discovery

Transposon tagging in maize

Social responsibility in human genetics – a survey

A genome-wide survey reveals a deletion polymorphism associated with resistance to gastrointestinal nematodes in Angus cattle

ES cell neural differentiation reveals a substantial number of novel ESTs