NDLI: Inducible Male Infertility by Targeted Cell Ablation in Zebrafish Testis

Content Provider	Springer Nature Link
Author	Hsu, Chia Chun Hou, Min Fon Hong, Jiann Ruey Wu, Jen Leih Her, Guor Mour
Copyright Year	2009
Abstract	To generate a zebrafish model of inducible male sterility, we expressed an Escherichia coli nitroreductase (Ntr) gene in the male germ line of zebrafish. The Ntr gene encodes an enzyme that can convert prodrugs such as metronidazole (Met) to cytotoxins. A fusion protein eGFP:Ntr (fusing Ntr to eGFP) under control of ∼ 2 kb putative promoters of the zebrafish testis-specific genes, A-kinase anchoring protein-associated protein (Asp), outer dense fibers (Odf), and sperm acrosomal membrane-associated protein (Sam) was expressed in the male germ line. Three independent and four compound transgenic zebrafish lines expressing eGFP:Ntr were established. Female carriers were fertile, while males exhibited different levels of sterility and appeared normal, otherwise. Developmental analysis shows that germ cells survived and testes were normal before Met treatment, but that the testes of all male transgenic zebrafish exhibited variously depleted prospermatogonia after Met treatment. Particularly in a triple-transgenic line, Tg(AOS-eGFP:Ntr)[Tg(Asp-eGFP:Ntr; Odf-eGFP:Ntr; Sam-eGFP:Ntr)], the transgenic males had very small testes that were virtually devoid of germ cells, and the residual germ cells had almost completely disappeared after 2 weeks of Met treatment. These zebrafish transgenic lines show the complete testis specificity of inducible male sterility after Met treatment and reveal a period of the Ntr/Met ablation activity just prior to formation of the definitive adult spermatogonial cell population. This study demonstrates that combined genetic and pharmacological methods for developing an “infertile breeding technology” have practical application in controlling genetically modified (GM) fish breeding and meet the standards of biological and environment safety for other GM species.
Starting Page	466
Ending Page	478
Page Count	13
File Format	PDF
ISSN	14362228
Journal	Marine Biotechnology
Volume Number	12
Issue Number	4
e-ISSN	14362236
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-11-24
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	GFP Transgenic fish Zebrafish Nitroreductase Metronidazole Male infertility Engineering Zoology Microbiology Freshwater & Marine Ecology
Content Type	Text
Resource Type	Article
Subject	Aquatic Science Biotechnology

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