NDLI: Production of Cold-Adapted Amylase by Marine Bacterium Wangia sp. C52: Optimization, Modeling, and Partial Characterization

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Production of Cold-Adapted Amylase by Marine Bacterium Wangia sp. C52: Optimization, Modeling, and Partial Characterization

Content Provider	Springer Nature Link
Author	Liu, Jianguo Zhang, Zhiqiang Liu, Zhiqiang Zhu, Hu Dang, Hongyue Lu, Jianren Cui, Zhanfeng
Copyright Year	2011
Abstract	The aim of this work was to optimize the fermentation parameters in the shake-flask culture of marine bacterium Wangia sp. C52 to increase cold-adapted amylase production using two statistical experimental methods including Plackett–Burman design, which was applied to find the key ingredients for the best medium composition, and response surface methodology, which was used to determine the optimal concentrations of these components. The results showed starch, tryptone, and initial pH had significant effects on the cold-adapted amylase production. A central composite design was then employed to further optimize these three factors. The experimental results indicated that the optimized composition of medium was 6.38 g L−1 starch, 33.84 g L−1 tryptone, 3.00 g L−1 yeast extract, 30 g L−1 NaCl, 0.60 g L−1 MgSO4 and 0.56 g L−1 CaCl2. The optimized cultivation conditions for amylase production were pH 7.18, a temperature of 20°C, and a shaking speed of 180 rpm. Under the proposed optimized conditions, the amylase experimental yield (676.63 U mL−1) closely matched the yield (685.60 U mL−1) predicted by the statistical model. The optimization of the medium contributed to tenfold higher amylase production than that of the control in shake-flask experiments.
Starting Page	837
Ending Page	844
Page Count	8
File Format	PDF
ISSN	14362228
Journal	Marine Biotechnology
Volume Number	13
Issue Number	5
e-ISSN	14362236
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-03-02
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cold-adapted amylase Optimization Plackett–Burman design Response surface methodology Wangia sp. Freshwater & Marine Ecology Microbiology Engineering Zoology
Content Type	Text
Resource Type	Article
Subject	Aquatic Science Biotechnology

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