NDLI: A New Method to Extract Matrix Proteins Directly from the Secretion of the Mollusk Mantle and the Role of These Proteins in Shell Biomineralization

Content Provider	Springer Nature Link
Author	Liu, Xiaojun Liu, Chang Chen, Lei Sun, Juan Zhou, Yujuan Li, Qi Zheng, Guilan Zhang, Guiyou Wang, Hongzhong Xie, Liping Zhang, Rongqing
Copyright Year	2011
Abstract	Considering the continuous and substantive secretory ability of the mantle in vitro, we report a new technique to produce shell-matrix proteins by inducing the mantle, after removal from the organism's body, to secrete soluble-matrix proteins into phosphate buffer. By this method, a large amount of matrix proteins could be obtained in 2 h. Experiments involving in vitro calcium carbonate crystallization and organic framework calcium carbonate crystallization indicated that these proteins retain high bioactivity and play key roles in shell biomineralization. Phosphate buffer–soluble proteins secreted by the margin of the mantles (MSPs) were used to reconstruct the stages in the growth of the prismatic layer of the decalcified organic frameworks. The MSPs were observed to aggregate calcites in vitro, and this ability enabled the mollusk to form big calcites in the prismatic layer. During shell biomineralization, an important stage after the self-assembly of the biomacromolecules and the formation of crystals is the assembly of the two parts to form a firm structure. Moreover, a new type of matrix protein, functioning as the binding factor between the crystals and the organic frameworks, was shown to exist in the phosphate buffer–soluble proteins secreted by the central part of mantles (CSPs). Nanoscale-sized bowl-like aragonites, with heights of ∼800 nm, were induced by CSPs in vitro. This method is a successful example of obtaining functional proteins through secretion by animal tissues.
Starting Page	981
Ending Page	991
Page Count	11
File Format	PDF
ISSN	14362228
Journal	Marine Biotechnology
Volume Number	13
Issue Number	5
e-ISSN	14362236
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-01-29
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Mollusks Pearl oyster Mantle secretion Shell-matrix proteins Biomineralization Freshwater & Marine Ecology Microbiology Engineering Zoology
Content Type	Text
Resource Type	Article
Subject	Aquatic Science Biotechnology

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