NDLI: Protection of Shrimp Penaeus monodon from WSSV Infection Using Antisense Constructs

Content Provider	Springer Nature Link
Author	Ahanger, Sajad Sandaka, Supriyanka Ananad, Deepika Mani, Madhu K. Kondadhasula, Ravinder Reddy, Chandra Sekhar Marappan, Makesh Valappil, Rajendran K. Majumdar, Kshitish C. Mishra, Rakesh K.
Copyright Year	2013
Abstract	White spot syndrome caused by white spot syndrome virus (WSSV) is one of the most threatening diseases of shrimp culture industry. Previous studies have successfully demonstrated the use of DNA- and RNA-based vaccines to protect WSSV infection in shrimp. In the present study, we have explored the protective efficacy of antisense constructs directed against WSSV proteins, VP24, and VP28, thymidylate synthase (TS), and ribonucleotide reductase-2 (RR2) under the control of endogenous shrimp histone-3 (H3) or penaedin (Pn) promoter. Several antisense constructs were generated by inserting VP24 (pH3–VP24, pPn–VP24), VP28 (pH3–VP28, pPn–VP28), TS (pH3–TS, pPn–TS), and RR2 (pH3–RR2) in antisense orientation. These constructs were tested for their protective potential in WSSV infected cell cultures, and their effect on reduction of the viral load was assessed. A robust reduction in WSSV copy number was observed upon transfection of antisense constructs in hemocyte cultures derived from Penaeus monodon and Scylla serrata. When tested in vivo, antisense constructs offered a strong protection in WSSV challenged P. monodon. Constructs expressing antisense VP24 and VP28 provided the best protection (up to 90 % survivability) with a corresponding decrease in the viral load. Our work demonstrates that shrimp treated with antisense constructs present an efficient control strategy for combating WSSV infection in shrimp aquaculture.
Starting Page	63
Ending Page	73
Page Count	11
File Format	PDF
ISSN	14362228
Journal	Marine Biotechnology
Volume Number	16
Issue Number	1
e-ISSN	14362236
Language	English
Publisher	Springer US
Publisher Date	2013-08-03
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	WSSV VP24 VP28 RNAi Antisense constructs Shrimp Freshwater & Marine Ecology Microbiology Zoology Engineering
Content Type	Text
Resource Type	Article
Subject	Aquatic Science Biotechnology

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