NDLI: Validation of Housekeeping Genes for Gene Expression Studies in Symbiodinium Exposed to Thermal and Light Stress

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Validation of Housekeeping Genes for Gene Expression Studies in Symbiodinium Exposed to Thermal and Light Stress

Content Provider	Springer Nature Link
Author	Rosic, Nedeljka N. Pernice, Mathieu Rodriguez Lanetty, Mauricio Hoegh Guldberg, Ove
Copyright Year	2010
Abstract	Unicellular photosynthetic algae (dinoflagellate) from the genus Symbiodinium live in mutualistic symbiosis with reef-building corals. Cultured Symbiodinium sp. (clade C) were exposed to a range of environmental stresses that included elevated temperatures (29°C and 32°C) under high (100 μmol quanta m−2 s−1 Photosynthetic Active Radiation) and low (10 μmol quanta m−2 s−1) irradiances. Using real-time RT-PCR the stability of expression for the nine selected putative housekeeping genes (HKGs) was tested. The most stable expression pattern was identified for cyclophilin and S-adenosyl methionine synthetase (SAM) followed by S4 ribosomal protein (Rp-S4), Calmodulin (Cal), and Cytochrome oxidase subunit 1 (Cox), respectively. Thermal stress alone resulted in the highest expression stability for Rp-S4 and SAM, with a minimum of two reference genes required for data normalization. For Symbiodinium exposed to both, light and thermal stresses, at least five reference genes were recommended by geNorm analysis. In parallel, the expression of Hsp90 for Symbiodinium in culture and in symbiosis within coral host (Acropora millepora) was evaluated using the most stable HKGs. Our results revealed a drop in Hsp90 expression after an 18 h-period and a 24 h-period of exposure to elevated temperatures indicating the similar Hsp90 expression profile in symbiotic and non-symbiotic environments. This study provides the first list of the HKGs and will provide a useful reference in future gene expression studies in symbiotic dinoflagellates.
Starting Page	355
Ending Page	365
Page Count	11
File Format	PDF
ISSN	14362228
Journal	Marine Biotechnology
Volume Number	13
Issue Number	3
e-ISSN	14362236
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-07-29
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Housekeeping genes Real-time RT-PCR Gene expression Dinoflagellate Climate change Freshwater & Marine Ecology Microbiology Engineering Zoology
Content Type	Text
Resource Type	Article
Subject	Aquatic Science Biotechnology

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