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| Content Provider | Springer Nature Link |
|---|---|
| Author | Parameswaran, V. Ishaq Ahmed, V. P. Shukla, Ravi Bhonde, R. R. Sahul Hameed, A. S. |
| Copyright Year | 2007 |
| Abstract | Two new cell lines, SIMH and SIGE, were derived from the heart of milkfish (Chanos chanos), a euryhaline teleost, and from the eye of grouper (Epinephelus coioides), respectively. These cell lines were maintained in Leibovitz’s L-15 supplemented with 20% fetal bovine serum (FBS). The SIMH cell line was subcultured more than 50 times over a period of 210 days and SIGE cell line has been subcultured 100 times over a period of 1 1/2 years. The SIMH cell line consists predominantly of fibroblastic-like cells. The SIGE cell line consists predominantly of epithelial cells. Both the cell lines were able to grow at temperatures between 25 and 32°C with an optimum temperature of 28°C. The growth rate of these cells increased as the proportion of FBS increased from 2% to 20% at 28°C with optimum growth at the concentrations of 15% or 20% FBS. Seven marine fish viruses were tested to determine the susceptibility of these cell lines. The SIGE cell line was found to be susceptible to nodavirus, MABV NC-1 and Y6, and the infection was confirmed by cytopathic effect (CPE) and reverse transcriptase-polymerase chain reaction. When these cells were transfected with pEGFP-N1 vector DNA, significant fluorescent signals were observed, suggesting that these cell lines can be a useful tool for transgenic and genetic manipulation studies. Further, these cell lines are characterized by immunocytochemistry using confocal laser scanning microscopy (CFLSM). |
| Starting Page | 281 |
| Ending Page | 291 |
| Page Count | 11 |
| File Format | |
| ISSN | 14362228 |
| Journal | Marine Biotechnology |
| Volume Number | 9 |
| Issue Number | 2 |
| e-ISSN | 14362236 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2007-01-10 |
| Publisher Place | New York |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | cell line Chanos chanos Epinephelus coioides eye heart virus Oceanography |
| Content Type | Text |
| Resource Type | Article |
| Subject | Aquatic Science Biotechnology |
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