NDLI: Real-Time Polymerase Chain Reaction Assays for Rapid Detection and Quantification of Noctiluca scintillans Zoospore

Please wait, while we are loading the content...

Marine Biotechnology : Volume 18

Marine Biotechnology : Volume 17

Marine Biotechnology : Volume 16

Marine Biotechnology : Volume 15

Marine Biotechnology : Volume 14

Marine Biotechnology : Volume 13

Marine Biotechnology : Volume 12

Marine Biotechnology : Volume 11

Marine Biotechnology : Volume 10

Marine Biotechnology : Volume 10, Issue 6, December 2008

Marine Biotechnology : Volume 10, Issue 5, September 2008

Marine Biotechnology : Volume 10, Issue 4, July 2008

Marine Biotechnology : Volume 10, Issue 3, May 2008

Marine Biotechnology : Volume 10, Issue 2, March 2008

Underwater Adhesive of Marine Organisms as the Vital Link Between Biological Science and Material Science

PCR Amplification of Microsatellites from Single Cells of Karenia brevis Preserved in Lugol’s Iodine Solution

Isolation and Characterization of Two Groups of Novel Marine Bacteria Producing Violacein

Real-Time Polymerase Chain Reaction Assays for Rapid Detection and Quantification of Noctiluca scintillans Zoospore

Mycosporine-Like Amino Acids Extracted from Scallop (Patinopecten yessoensis) Ovaries: UV Protection and Growth Stimulation Activities on Human Cells

FISH Mapping and Identification of Zhikong Scallop (Chlamys farreri) Chromosomes

Induction of Attachment of the Mussel Perna perna by Natural Products from the Brown Seaweed Stypopodium zonale

Gene Expression of a Green Fluorescent Protein Homolog as a Host-Specific Biomarker of Heat Stress Within a Reef-Building Coral

A Novel Approach for Bioremediation of a Coastal Marine Wastewater Effluent Based on Artificial Microbial Mats

Purification and Characterization of Extracellular Phytase from a Marine Yeast Kodamaea ohmeri BG3

Silencing VP28 Gene of White Spot Syndrome Virus of Shrimp by Bacterially Expressed dsRNA

DNA Microarrays for Identifying Fishes

Marine Biotechnology : Volume 10, Issue 1, January 2008

Marine Biotechnology : Volume 9

Marine Biotechnology : Volume 8

Marine Biotechnology : Volume 7

Marine Biotechnology : Volume 6

Marine Biotechnology : Volume 5

Marine Biotechnology : Volume 4

Marine Biotechnology : Volume 3

Marine Biotechnology : Volume 2

Marine Biotechnology : Volume 1

Real-Time Polymerase Chain Reaction Assays for Rapid Detection and Quantification of Noctiluca scintillans Zoospore

Content Provider	Springer Nature Link
Author	Miyaguchi, Hideo Kurosawa, rio Toda, Tatsuki
Copyright Year	2007
Abstract	Application and availability of real-time polymerase chain reaction (PCR) assay to detect and quantify the Noctiluca scintillans zoospore were investigated seasonally. Specific primer set for N. scintillans 18S rDNA was designed and applied to real-time PCR assay using the serial dilutions of N. scintillans zoospores. The real-time PCR assays with Ns63F and Ns260R primers were applied to sea water samples collected weekly in Manazuru Port of Sagami Bay, Japan from April 2005 to June 2006. We developed effective DNA preparation steps for collecting the template DNA of N. scintillans zoospore: size fraction and filter concentration of the water samples, fixation with Lugol solution, cell lysis, and purification. This method is useful for the monitoring of the zoospores of N. scintillans, and can also be used for other small and physiologically fragile planktonic cell. Variation in the density of zoospore was successfully detected in the field samples. The peak density of N. scintillans zoospore was observed to occur just before or at the same time as the peak of the vegetative cells. Moreover, zoospores were detected in seawater even when the vegetative cells were not observed. The presence of zoospore was found all year round in the present study. In this regards, this information is essential for the study of the life cycle and seasonal variation of N. scintillans in the coastal waters.
Starting Page	133
Ending Page	140
Page Count	8
File Format	PDF
ISSN	14362228
Journal	Marine Biotechnology
Volume Number	10
Issue Number	2
e-ISSN	14362236
Language	English
Publisher	Springer-Verlag
Publisher Date	2007-10-05
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	dinoflagellate Noctiluca scintillans real-time PCR red tide Sagami Bay zoospore Engineering Zoology Microbiology Freshwater & Marine Ecology
Content Type	Text
Resource Type	Article
Subject	Aquatic Science Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Seasonal variability of the red tide-forming heterotrophic dinoflagellate Noctiluca scintillans in the neritic area of Sagami Bay, Japan: its role in the nutrient-environment and aquatic ecosystem

The influence of upwelling, coastal currents and water temperature on the distribution of the red tide dinoflagellate, Noctiluca scintillans, along the east coast of Australia

Phytoplankton-Group Specific Quantitative Polymerase Chain Reaction Assays for RuBisCO mRNA Transcripts in Seawater

Development of a quantification assay for the cysts of the toxic dinoflagellate Alexandrium tamarense using real-time polymerase chain reaction

Hydrographical and Bio-ecological Characteristics of Heterotrophic Red Tide Dinoflagellate Noctiluca scintillans in Semi-enclosed Gwangyang Bay, Korea

Cloning of proliferating cell nuclear antigen gene from the dinoflagellate Prorocentrum donghaiense and monitoring its expression profiles by real-time RT-PCR

Red tides of the dinoflagellate Noctiluca scintillans associated with eutrophication in the Sea of Marmara (the Dardanelles, Turkey)

3-D observations of a red tide event in the offshore water along the western Guangdong coast

Diurnal variability of Synechococcus abundance in Sagami Bay, Japan

Real-Time Polymerase Chain Reaction Assays for Rapid Detection and Quantification of Noctiluca scintillans Zoospore

Similar Documents

Seasonal variability of the red tide-forming heterotrophic dinoflagellate Noctiluca scintillans in the neritic area of Sagami Bay, Japan: its role in the nutrient-environment and aquatic ecosystem

The influence of upwelling, coastal currents and water temperature on the distribution of the red tide dinoflagellate, Noctiluca scintillans, along the east coast of Australia

Phytoplankton-Group Specific Quantitative Polymerase Chain Reaction Assays for RuBisCO mRNA Transcripts in Seawater

Development of a quantification assay for the cysts of the toxic dinoflagellate Alexandrium tamarense using real-time polymerase chain reaction

Hydrographical and Bio-ecological Characteristics of Heterotrophic Red Tide Dinoflagellate Noctiluca scintillans in Semi-enclosed Gwangyang Bay, Korea

Cloning of proliferating cell nuclear antigen gene from the dinoflagellate Prorocentrum donghaiense and monitoring its expression profiles by real-time RT-PCR

Red tides of the dinoflagellate Noctiluca scintillans associated with eutrophication in the Sea of Marmara (the Dardanelles, Turkey)

3-D observations of a red tide event in the offshore water along the western Guangdong coast

Diurnal variability of Synechococcus abundance in Sagami Bay, Japan

Real-Time Polymerase Chain Reaction Assays for Rapid Detection and Quantification of Noctiluca scintillans Zoospore