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  1. European Journal of Clinical Microbiology & Infectious Diseases
  2. European Journal of Clinical Microbiology & Infectious Diseases : Volume 34
  3. European Journal of Clinical Microbiology & Infectious Diseases : Volume 34, Issue 9, September 2015
  4. Rapid urine preparation prior to identification of uropathogens by MALDI-TOF MS
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European Journal of Clinical Microbiology & Infectious Diseases : Volume 36
European Journal of Clinical Microbiology & Infectious Diseases : Volume 35
European Journal of Clinical Microbiology & Infectious Diseases : Volume 34
European Journal of Clinical Microbiology & Infectious Diseases : Volume 34, Issue 12, December 2015
European Journal of Clinical Microbiology & Infectious Diseases : Volume 34, Issue 11, November 2015
European Journal of Clinical Microbiology & Infectious Diseases : Volume 34, Issue 10, October 2015
European Journal of Clinical Microbiology & Infectious Diseases : Volume 34, Issue 9, September 2015
Molecular mechanisms underlying HBsAg negativity in occult HBV infection
Molecular studies on ancient M. tuberculosis and M. leprae: methods of pathogen and host DNA analysis
Impact of borderline minimum inhibitory concentration on the outcome of invasive infections caused by Enterobacteriaceae treated with β-lactams: a systematic review and meta-analysis
Prevalence of chronic pulmonary aspergillosis in patients with tuberculosis from Iran
Fusarium spp. is able to grow and invade healthy human nails as a single source of nutrients
Neonatal urinary tract infection may increase the risk of childhood asthma
A multiplex real-time PCR assay for routine diagnosis of bacterial vaginosis
Rapid urine preparation prior to identification of uropathogens by MALDI-TOF MS
Decline in macrolide resistance rates among Streptococcus pyogenes causing pharyngitis in children isolated in Italy
Comparison of Sofia Legionella FIA and BinaxNOW® Legionella urinary antigen card in two national reference centers
Prolonging culture to 15 days improves bacterial detection in bone and joint infections
Fluoroquinolone-based antimicrobial prophylaxis in patients undergoing transrectal ultrasound-guided prostate biopsy
Colonisation of dentures by Staphylococcus aureus and MRSA in out-patient and in-patient populations
Performance of the new automated Abbott RealTime MTB assay for rapid detection of Mycobacterium tuberculosis complex in respiratory specimens
No change in the distribution of types and antibiotic resistance in clinical Staphylococcus aureus isolates from orthopaedic patients during a period of 12 years
Impact of bacterial coinfection on clinical outcomes in pneumococcal pneumonia
Impact of round-the-clock CSF Gram stain on empirical therapy for suspected central nervous system infections
Evaluation of the PREVI® Isola automated seeder system compared to reference manual inoculation for antibiotic susceptibility testing by the disk diffusion method
Fecal detection of enterotoxigenic Bacteroides fragilis
Efficacy of and risk of bleeding during pegylated interferon plus ribavirin treatment in HIV/HCV-coinfected patients with pretreatment thrombocytopenia
Direct molecular versus culture-based assessment of Gram-positive cocci in biopsies of patients with major abscesses and diabetic foot infections
A three-point time series study of antibiotic usage on an intensive care unit, following an antibiotic stewardship programme, after an outbreak of multi-resistant Acinetobacter baumannii
Environmental survival of vancomycin-sensitive ampicillin-resistant Enterococcus faecium (AREfm)
Evaluation of the R-Biopharm RIDA®GENE Panton–Valentine leukocidin (PVL) kit for the detection of Staphylococcus aureus PVL from pus samples
Clinical findings in relation to mortality in non-tuberculous mycobacterial infections: patients with Mycobacterium avium complex have better survival than patients with other mycobacteria
−295 T-to-C promoter region IL-16 gene polymorphism is associated with Whipple’s disease
European Journal of Clinical Microbiology & Infectious Diseases : Volume 34, Issue 8, August 2015
European Journal of Clinical Microbiology & Infectious Diseases : Volume 34, Issue 7, July 2015
European Journal of Clinical Microbiology & Infectious Diseases : Volume 34, Issue 6, June 2015
European Journal of Clinical Microbiology & Infectious Diseases : Volume 34, Issue 5, May 2015
European Journal of Clinical Microbiology & Infectious Diseases : Volume 34, Issue 4, April 2015
European Journal of Clinical Microbiology & Infectious Diseases : Volume 34, Issue 3, March 2015
European Journal of Clinical Microbiology & Infectious Diseases : Volume 34, Issue 2, February 2015
European Journal of Clinical Microbiology & Infectious Diseases : Volume 34, Issue 1, January 2015
European Journal of Clinical Microbiology & Infectious Diseases : Volume 33
European Journal of Clinical Microbiology & Infectious Diseases : Volume 32
European Journal of Clinical Microbiology & Infectious Diseases : Volume 31
European Journal of Clinical Microbiology & Infectious Diseases : Volume 30
European Journal of Clinical Microbiology & Infectious Diseases : Volume 29
European Journal of Clinical Microbiology & Infectious Diseases : Volume 28
European Journal of Clinical Microbiology & Infectious Diseases : Volume 27
European Journal of Clinical Microbiology & Infectious Diseases : Volume 26
European Journal of Clinical Microbiology & Infectious Diseases : Volume 25
European Journal of Clinical Microbiology & Infectious Diseases : Volume 24
European Journal of Clinical Microbiology & Infectious Diseases : Volume 23
European Journal of Clinical Microbiology & Infectious Diseases : Volume 22
European Journal of Clinical Microbiology & Infectious Diseases : Volume 21
European Journal of Clinical Microbiology & Infectious Diseases : Volume 20
European Journal of Clinical Microbiology & Infectious Diseases : Volume 19
European Journal of Clinical Microbiology & Infectious Diseases : Volume 18
European Journal of Clinical Microbiology & Infectious Diseases : Volume 17
European Journal of Clinical Microbiology & Infectious Diseases : Volume 16

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Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) for rapid identification of micro-organisms in the routine clinical microbiology laboratory

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Rapid urine preparation prior to identification of uropathogens by MALDI-TOF MS

Content Provider Springer Nature Link
Author Veron, L. Mailler, S. Girard, V. Muller, B. H. L’Hostis, G. Ducruix, C. Lesenne, A. Richez, A. Rostaing, H. Lanet, V. Ghirardi, S. Belkum, A. Mallard, F.
Copyright Year 2015
Abstract Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-ToF MS) has been introduced in clinical routine microbiology laboratories. For the rapid diagnosis of urinary tract infections, culture-independent methods prior MALDI-mediated identification have been described. Here, we describe a comparison of three of these methods based on their performance of bacterial identification and their potential as a routine tool for microbiology labs : (i) differential centrifugation, (ii) urine filtration and (iii) a 5-h bacterial cultivation on solid culture media. For 19 urine samples, all methods were directly compared and correct bacterial species identification by MALDI was used as performance indicator. A higher percentage of correct MALDI identification was obtained after filtration (78.9 %) and the growth-based method (84.2 %) as compared to differential centrifugation (68.4 %). Additional testing of 76 mono-microbial specimens (bacteriuria > 105 CFU/mL) confirmed the good performance of short growth with a 90.8 % correct MALDI score, with a potentially better fit to the routine workflow of microbiology labs.
Starting Page 1787
Ending Page 1795
Page Count 9
File Format PDF
ISSN 09349723
Journal European Journal of Clinical Microbiology & Infectious Diseases
Volume Number 34
Issue Number 9
e-ISSN 14354373
Language English
Publisher Springer Berlin Heidelberg
Publisher Date 2015-06-09
Publisher Place Berlin, Heidelberg
Access Restriction One Nation One Subscription (ONOS)
Subject Keyword Medical Microbiology Internal Medicine
Content Type Text
Resource Type Article
Subject Infectious Diseases Microbiology (medical)
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