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  1. European Journal of Clinical Microbiology & Infectious Diseases
  2. European Journal of Clinical Microbiology & Infectious Diseases : Volume 18
  3. European Journal of Clinical Microbiology & Infectious Diseases : Volume 18, Issue 6, July 1999
  4. Validity of Screening Procedures for Glycopeptide-Resistant Enterococci
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European Journal of Clinical Microbiology & Infectious Diseases : Volume 36
European Journal of Clinical Microbiology & Infectious Diseases : Volume 35
European Journal of Clinical Microbiology & Infectious Diseases : Volume 34
European Journal of Clinical Microbiology & Infectious Diseases : Volume 33
European Journal of Clinical Microbiology & Infectious Diseases : Volume 32
European Journal of Clinical Microbiology & Infectious Diseases : Volume 31
European Journal of Clinical Microbiology & Infectious Diseases : Volume 30
European Journal of Clinical Microbiology & Infectious Diseases : Volume 29
European Journal of Clinical Microbiology & Infectious Diseases : Volume 28
European Journal of Clinical Microbiology & Infectious Diseases : Volume 27
European Journal of Clinical Microbiology & Infectious Diseases : Volume 26
European Journal of Clinical Microbiology & Infectious Diseases : Volume 25
European Journal of Clinical Microbiology & Infectious Diseases : Volume 24
European Journal of Clinical Microbiology & Infectious Diseases : Volume 23
European Journal of Clinical Microbiology & Infectious Diseases : Volume 22
European Journal of Clinical Microbiology & Infectious Diseases : Volume 21
European Journal of Clinical Microbiology & Infectious Diseases : Volume 20
European Journal of Clinical Microbiology & Infectious Diseases : Volume 19
European Journal of Clinical Microbiology & Infectious Diseases : Volume 18
European Journal of Clinical Microbiology & Infectious Diseases : Volume 18, Issue 12, December 1999
European Journal of Clinical Microbiology & Infectious Diseases : Volume 18, Issue 11, December 1999
European Journal of Clinical Microbiology & Infectious Diseases : Volume 18, Issue 10, November 1999
European Journal of Clinical Microbiology & Infectious Diseases : Volume 18, Issue 9, October 1999
European Journal of Clinical Microbiology & Infectious Diseases : Volume 18, Issue 8, September 1999
European Journal of Clinical Microbiology & Infectious Diseases : Volume 18, Issue 7, August 1999
European Journal of Clinical Microbiology & Infectious Diseases : Volume 18, Issue 6, July 1999
Molecular Characterization and Transfer Among Staphylococcus Strains of a Plasmid Conferring High-Level Resistance to Mupirocin
Rare AIDS-Defining Diseases in the Swiss HIV Cohort Study
Frequency of Occurrence and Antimicrobial Susceptibility of Bacterial Pathogens Associated with Skin and Soft Tissue Infections During 1997 from an International Surveillance Programme
Incidence of Haemophilus influenzae Type b Meningitis Among Children Less Than 5 Years of Age in Slovakia
Prevalence of Aminoglycoside Resistance in 20 European University Hospitals Participating in the European SENTRY Antimicrobial Surveillance Programme
Validity of Screening Procedures for Glycopeptide-Resistant Enterococci
Comparative In Vitro Activity of Gatifloxacin Against Stenotrophomonas maltophilia and Burkholderia Species Isolates Including Evaluation of Disk Diffusion and E Test Methods
Comparative in Vitro Activity of Voriconazole and Itraconazole Against Fluconazole-Susceptible and Fluconazole-Resistant Clinical Isolates of Candida Species from Spain
Irritable Bowel Syndrome in Patients with Blastocystis hominis Infection
Five-Year Analysis of Antimicrobial Susceptibility of the Streptococcus milleri Group
Detection of Legionella DNA in Guinea Pig Peripheral Leukocytes, Urine and Plasma by the Polymerase Chain Reaction
Usefulness of Genotypic Analysis of Resistance to Nucleoside Analogues in the Clinical Setting
Comparative Activity of 27 Antimicrobial Compounds Against 698 Streptococcus pneumoniae Isolates Originating from 20 European University Hospitals
Candida Meningitis Possibly Resulting from a Harpoon Injury
Carbapenem-Hydrolyzing Metallo-Beta-Lactamases in Pseudomonas aeruginosa
Three Cases of Pneumonia due to Neisseria meningitidis, Including Serogroup W135
Lack of Association Between GBV-C Infection and Spontaneous Abortion
Announcements
European Journal of Clinical Microbiology & Infectious Diseases : Volume 18, Issue 5, June 1999
European Journal of Clinical Microbiology & Infectious Diseases : Volume 18, Issue 4, May 1999
European Journal of Clinical Microbiology & Infectious Diseases : Volume 18, Issue 3, April 1999
European Journal of Clinical Microbiology & Infectious Diseases : Volume 18, Issue 2, March 1999
European Journal of Clinical Microbiology & Infectious Diseases : Volume 18, Issue 1, February 1999
European Journal of Clinical Microbiology & Infectious Diseases : Volume 17
European Journal of Clinical Microbiology & Infectious Diseases : Volume 16

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Validity of Screening Procedures for Glycopeptide-Resistant Enterococci

Content Provider Springer Nature Link
Author Wendt, C. Krause, C. Floss, H.
Copyright Year 1999
Abstract Screening agars containing different vancomycin concentrations and different susceptibility testing procedures were compared to determine their validity for the detection of glycopeptide-resistant enterococci (GRE). Direct streaking of rectal swabs over the surface of a commercially available agar (Enterococcosel; Becton Dickinson, Germany) containing 4 μg/ml and 16 μg/ml vancomycin was followed by incubation for 24 to 48 h. Susceptibility tests were done by microbroth dilution, disk diffusion, and the E test (AB Biodisk, Sweden). The microbroth dilution method according to National Committee for Clinical Laboratory Standards (NCCLS) was used as the gold standard for detection of GRE. Resistant and intermediately susceptible enterococcal isolates were differentiated to the species level. To detect resistance genes, the polymerase chain reaction was performed on all intermediately resistant isolates, on all isolates of VanB phenotype, and on 30% of isolates of VanA phenotype. Screening agar containing 4 μg/ml vancomycin displayed high sensitivity (97.6%) but only low specificity (35%) for the detection of GRE. Screening agar containing 16 μg/ml vancomycin had a high specificity of 89.3% and only a slightly lower sensitivity (92.7%) than the screening agar containing 4 μg/ml vancomycin. For the disk diffusion test, a breakpoint of <16 mm yielded the optimal combination of sensitivity (98.8%) and specificity (99.6%). Both sensitivity and specificity of the E test for GRE detection were 100%. However, the E test is too expensive for testing of all enterococci. In conclusion, the combination of an inexpensive screening agar with either the E test or the disk diffusion test constitutes a valid and cost-effective method for the detection of GRE from screening specimens.
Starting Page 422
Ending Page 427
Page Count 6
File Format PDF
ISSN 09349723
Journal European Journal of Clinical Microbiology & Infectious Diseases
Volume Number 18
Issue Number 6
e-ISSN 14354373
Language English
Publisher Springer-Verlag
Publisher Date 1999-07-02
Publisher Place Berlin, Heidelberg
Access Restriction One Nation One Subscription (ONOS)
Content Type Text
Resource Type Article
Subject Infectious Diseases Microbiology (medical)
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