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  1. European Journal of Clinical Microbiology & Infectious Diseases
  2. European Journal of Clinical Microbiology & Infectious Diseases : Volume 33
  3. European Journal of Clinical Microbiology & Infectious Diseases : Volume 33, Issue 1, January 2014
  4. Escherichia coli biofilm formation and recurrences of urinary tract infections in children
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European Journal of Clinical Microbiology & Infectious Diseases : Volume 36
European Journal of Clinical Microbiology & Infectious Diseases : Volume 35
European Journal of Clinical Microbiology & Infectious Diseases : Volume 34
European Journal of Clinical Microbiology & Infectious Diseases : Volume 33
European Journal of Clinical Microbiology & Infectious Diseases : Volume 33, Issue 12, December 2014
European Journal of Clinical Microbiology & Infectious Diseases : Volume 33, Issue 11, November 2014
European Journal of Clinical Microbiology & Infectious Diseases : Volume 33, Issue 10, October 2014
European Journal of Clinical Microbiology & Infectious Diseases : Volume 33, Issue 9, September 2014
European Journal of Clinical Microbiology & Infectious Diseases : Volume 33, Issue 8, August 2014
European Journal of Clinical Microbiology & Infectious Diseases : Volume 33, Issue 7, July 2014
European Journal of Clinical Microbiology & Infectious Diseases : Volume 33, Issue 6, June 2014
European Journal of Clinical Microbiology & Infectious Diseases : Volume 33, Issue 5, May 2014
European Journal of Clinical Microbiology & Infectious Diseases : Volume 33, Issue 4, April 2014
European Journal of Clinical Microbiology & Infectious Diseases : Volume 33, Issue 3, March 2014
European Journal of Clinical Microbiology & Infectious Diseases : Volume 33, Issue 2, February 2014
European Journal of Clinical Microbiology & Infectious Diseases : Volume 33, Issue 1, January 2014
Antibiotic prophylaxis in children with cancer or who have undergone hematopoietic cell transplantation
Clinical and economic burden of invasive fungal diseases in Europe: focus on pre-emptive and empirical treatment of Aspergillus and Candida species
Gonococcal endocarditis: a case report and literature review
Reliability of the MicroScan WalkAway PC21 panel in identifying and detecting oxacillin resistance in clinical coagulase-negative staphylococci strains
Performance of chromID® CARBA medium for carbapenemases-producing enterobacteriaceae detection during rectal screening
Impact of rhinovirus nasopharyngeal viral load and viremia on severity of respiratory infections in children
Purification of DNA eluates from the ProbeTec GC Qx assay on BD Viper™ XTR allows for further analysis and confirmation of gonorrhea
Risk factors for levofloxacin-nonsusceptible Streptococcus pneumoniae in community-acquired pneumococcal pneumonia: a nested case–control study
Epidemiology of MRSA in southern Sweden: strong relation to foreign country of origin, health care abroad and foreign travel
Surface antigen-negative hepatitis B virus infection in Dutch blood donors
Epidemic increase in Salmonella bloodstream infection in children, Bwamanda, the Democratic Republic of Congo
Dutch guideline on the laboratory detection of methicillin-resistant Staphylococcus aureus
Study of the hormetic effect of disinfectants chlorhexidine, povidone iodine and benzalkonium chloride
Escherichia coli biofilm formation and recurrences of urinary tract infections in children
Macrophage-related serum biomarkers soluble CD163 (sCD163) and soluble mannose receptor (sMR) to differentiate mild liver fibrosis from cirrhosis in patients with chronic hepatitis C: a pilot study
Smoking increases oral HPV persistence among men: 7-year follow-up study
Treatment and outcomes of Candida osteomyelitis: review of 53 cases from the PATH Alliance® registry
Acknowledgement to referees
European Journal of Clinical Microbiology & Infectious Diseases : Volume 32
European Journal of Clinical Microbiology & Infectious Diseases : Volume 31
European Journal of Clinical Microbiology & Infectious Diseases : Volume 30
European Journal of Clinical Microbiology & Infectious Diseases : Volume 29
European Journal of Clinical Microbiology & Infectious Diseases : Volume 28
European Journal of Clinical Microbiology & Infectious Diseases : Volume 27
European Journal of Clinical Microbiology & Infectious Diseases : Volume 26
European Journal of Clinical Microbiology & Infectious Diseases : Volume 25
European Journal of Clinical Microbiology & Infectious Diseases : Volume 24
European Journal of Clinical Microbiology & Infectious Diseases : Volume 23
European Journal of Clinical Microbiology & Infectious Diseases : Volume 22
European Journal of Clinical Microbiology & Infectious Diseases : Volume 21
European Journal of Clinical Microbiology & Infectious Diseases : Volume 20
European Journal of Clinical Microbiology & Infectious Diseases : Volume 19
European Journal of Clinical Microbiology & Infectious Diseases : Volume 18
European Journal of Clinical Microbiology & Infectious Diseases : Volume 17
European Journal of Clinical Microbiology & Infectious Diseases : Volume 16

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Escherichia coli biofilm formation and recurrences of urinary tract infections in children

Content Provider Springer Nature Link
Author Tapiainen, T. Hanni, A. M. Salo, J. Ikäheimo, I. Uhari, M.
Copyright Year 2013
Abstract It has been suggested that biofilm formation by uropathogenic Escherichia coli (UPEC) isolates is associated with recurrence and persistence of urinary tract infection (UTI). We compared the in vitro biofilm formation of UPEC isolates from children with acute or recurrent UTI. Employing 206 consecutive clinical UPEC isolates from children with proven UTI, i.e., pyelonephritis (n = 78), recurrent pyelonephritis (n = 10), cystitis (n = 84) or recurrent cystitis (n = 34), we applied 1 % crystal violet staining to polystyrene microtitre plates at 72 h and measured the optical density (OD) values. The method had been validated to measure biofilm formation against confocal laser scanning microscopy and scanning electron microscopy. The OD values were lower in the recurrent cystitis group than in the other groups (mean OD 0.36, SD 0.21 vs mean 0.47, SD 0.36, P = 0.04) and higher in the recurrent pyelonephritis group than in the other groups (mean OD 0.69, SD 0.33 vs mean OD 0.44, SD 0.34, P = 0.006) indicating biofilm formation of strains causing recurrent pyelonephritis. It appears that the properties of UPEC isolates required for effective biofilm growth on an abiotic surface are important for recurrent pyelonephritis, but not for recurrent cystitis. It would be valuable in the future to analyze whether the biofilm properties of E. coli observed in vitro predict a slower clinical response to antimicrobial treatment and increased renal scar formation after UTI.
Starting Page 111
Ending Page 115
Page Count 5
File Format PDF
ISSN 09349723
Journal European Journal of Clinical Microbiology & Infectious Diseases
Volume Number 33
Issue Number 1
e-ISSN 14354373
Language English
Publisher Springer Berlin Heidelberg
Publisher Date 2013-09-02
Publisher Place Berlin, Heidelberg
Access Restriction One Nation One Subscription (ONOS)
Subject Keyword Medical Microbiology Internal Medicine
Content Type Text
Resource Type Article
Subject Infectious Diseases Microbiology (medical)
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