NDLI: Effect of Pinus radiate bark extracts with different molecular weight distributions on cell growth of NIH/3T3 fibroblasts and dendrite retraction of B16 melanoma cells

Content Provider	Springer Nature Link
Author	Ku, Chang Sub Mun, Sung Phil
Copyright Year	2011
Abstract	Hot water extract (HWE) of Pinus radiata bark was separated into monomeric polyphenol (MPP), oligomeric proanthocyanidin (OPA), and polymeric proanthocyanidin (PPA) fractions by monitored chromatography using a Sephadex LH 20 column and an UV detector at 250 nm. The effects of these fractions on NIH/3T3 fibroblasts and B16 melanoma cells were examined by evaluating cell viability, melanogenesis (melanin content), morphological changes, and tyrosinase inhibitory activity. The polyphenolic fractions had a proliferation effect on fibroblasts, with cell growth increasing significantly (P < 0.01) even at the high concentration of 1250 μg/ml. At 125 μg/ml, HWE, MPP, and OPA had no effect on melanogenesis, whereas PPA significantly upregulated melanogenesis (P < 0.05). Melanogenesis was significantly upregulated in melanoma cells treated with these fractions at the high concentration of 600 μg/ml (P < 0.01). B16 melanoma cells cultured with the proanthocyanidin (PA)-rich fractions (HWE, OPA, and PPA) showed marked dendrite retraction, leading to significant morphological transformation. OPA in particular showed colored adhesion on the surface of melanoma cells. All four fractions significantly inhibited mushroom tyrosinase activity when compared to arbutin and ascorbic acid 2-glucoside (P < 0.05). The tyrosinase inhibitory activity of OPA and PPA did not differ significantly (P = 0.11), indicating that the inhibitory effects had a low correlation with molecular weight distribution. The inhibition kinetics of PPA determined using a Lineweaver-Burk plot indicated that PPA is a noncompetitive inhibitor of l-3,4-dihydroxyphenylalanine oxidation by mushroom tyrosinase.
Starting Page	415
Ending Page	420
Page Count	6
File Format	PDF
ISSN	14350211
Journal	Journal of Wood Science
Volume Number	57
Issue Number	5
e-ISSN	16114663
Language	English
Publisher	Springer Japan
Publisher Date	2011-07-16
Publisher Place	Japan
Access Restriction	Subscribed
Subject Keyword	Pinus radiata bark Molecular weight distribution NIH/3T3 fibroblasts Dendrite retraction B16 melanoma cell Materials Science Wood Science & Technology Characterization and Evaluation of Materials
Content Type	Text
Resource Type	Article
Subject	Biomaterials Forestry

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