NDLI: Antioxidant activity of phenolic compounds from canola (Brassica napus) seed

Content Provider	Springer Nature Link
Author	Jun, Hyun Il Wiesenborn, Dennis P. Kim, Young Soo
Copyright Year	2014
Abstract	The antioxidant activities of various extracts from canola (Brassica napus) seed were investigated using the DPPH assay, ABTS radical assay, and reducing power. An 80% methanol extract from canola seed was sequentially fractionated and separated according to the solvent polarity and Sephadex LH-20 chromatography, respectively. The antioxidant activities of various extracts and their total phenolic content (TPC) and total flavonoid content (TFC) were closely correlated, resulting in correlation coefficient values higher than 0.87. Of all extracts, the sub-fraction 3 had the highest TPC (462.3 μg/mg) and TFC (75.4 μg/mg) while it showed the lowest EC50 value (183.1 μg/mL). The EC50 value of sub-fraction 3 measured using the DPPH radical assay was 1.3 times lower than that of butylated hydroxytoluene (BHT). The major free phenolic compound was trans-sinapic acid (193.4 μg/mg) and sinapic acid derivatives identified as 1-O-β-d-glucopyranosyl sinapate and 1,2-di-O-sinapoyl-β-d-glucose in sub-fraction 3.
Starting Page	1753
Ending Page	1760
Page Count	8
File Format	PDF
ISSN	12267708
Journal	Food Science and Biotechnology
Volume Number	23
Issue Number	6
e-ISSN	20926456
Language	English
Publisher	The Korean Society of Food Science and Technology
Publisher Date	2014-12-31
Publisher Place	Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	canola seed antioxidant activity phenolic compound sinapic acid sinapic acid derivatives Food Science Nutrition
Content Type	Text
Resource Type	Article
Subject	Applied Microbiology and Biotechnology Food Science Biotechnology

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