NDLI: Detection of Campylobacter DNA using magnetic nanoparticles coupled with PCR and a colorimetric end-point system

Content Provider	Springer Nature Link
Author	Jansaento, Wuttichote Jangpatarapongsa, Kulachart Polpanich, Duangporn Wonglumsom, Wijit
Copyright Year	2016
Abstract	Campylobacter is an important food-borne pathogen causing acute gastroenteritis worldwide. Magnetic nanoparticle-based PCR coupled with streptavidin-horseradish peroxidase and a substrate was used for colorimetric detection. Forward primers conjugated to magnetic nanoparticles facilitated separation and concentration of Campylobacter DNA in a sample matrix. After PCR, a green color developed and was observed using the unaided eye, or detected using a spectrophotometer. High specificity and sensitivity of the 100 fg DNA/PCR reaction were achieved in pure culture experiments. The technique was applied for detection of Campylobacter on naturally contaminated chicken skin. All positive results were in agreement with results achieved using a conventional culture method. The magnetic nanoparticle-PCR-enzyme linked gene assay was practical and useful for detection of Campylobacter in complex matrices with PCR-interfering substances.
Starting Page	193
Ending Page	198
Page Count	6
File Format	PDF
ISSN	12267708
Journal	Food Science and Biotechnology
Volume Number	25
Issue Number	1
e-ISSN	20926456
Language	English
Publisher	The Korean Society of Food Science and Technology
Publisher Date	2016-02-29
Publisher Place	Seoul
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Campylobacter magnetic nanoparticle colorimetric system chicken Food Science Nutrition
Content Type	Text
Resource Type	Article
Subject	Applied Microbiology and Biotechnology Food Science Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Simultaneous Detection and Differentiation of Campylobacter jejuni, C. coli, and C. lari in Chickens Using a Multiplex Real-Time PCR Assay

Direct Quantification of Campylobacter jejuni in Chicken Fecal Samples Using Real-Time PCR: Evaluation of Six Rapid DNA Extraction Methods

A Novel Colorimetric Detection of S. typhimurium Based on Fe$_{3}$O$_{4}$ Magnetic Nanoparticles and Gold Nanoparticles

Gold nanoparticles-based colorimetric assay for rapid detection of Salmonella species in food samples

Comparison of competitive and positive control-based PCR quantitative procedures coupled with end point detection

Event-specific detection system of stacked genetically modified maize by using the multiplex-PCR technique

Detection system of stacked genetically modified maize using multiplex PCR

A new RAPD-PCR based analytical assay for detection of sea bass and sea bream treated with ionizing radiation

Rapid detection of Campylobacter jejuni using fluorescent microspheres as label for immunochromatographic strip test

Detection of Campylobacter DNA using magnetic nanoparticles coupled with PCR and a colorimetric end-point system

Similar Documents

Simultaneous Detection and Differentiation of Campylobacter jejuni, C. coli, and C. lari in Chickens Using a Multiplex Real-Time PCR Assay

Direct Quantification of Campylobacter jejuni in Chicken Fecal Samples Using Real-Time PCR: Evaluation of Six Rapid DNA Extraction Methods

A Novel Colorimetric Detection of S. typhimurium Based on Fe$_{3}$O$_{4}$ Magnetic Nanoparticles and Gold Nanoparticles

Gold nanoparticles-based colorimetric assay for rapid detection of Salmonella species in food samples

Comparison of competitive and positive control-based PCR quantitative procedures coupled with end point detection

Event-specific detection system of stacked genetically modified maize by using the multiplex-PCR technique

Detection system of stacked genetically modified maize using multiplex PCR

A new RAPD-PCR based analytical assay for detection of sea bass and sea bream treated with ionizing radiation

Rapid detection of Campylobacter jejuni using fluorescent microspheres as label for immunochromatographic strip test

Detection of Campylobacter DNA using magnetic nanoparticles coupled with PCR and a colorimetric end-point system